ArticleMolecular biology of the cell2015
Pat1 protects centromere-specific histone H3 variant Cse4 from Psh1-mediated ubiquitination.
Article in Molecular biology of the cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 26 citations in OpenAlex.
- Keeping it centered: decoding the activities that regulate yeast histone H3 variant Cse4 and confine it to centromeres.Frontiers in cell and developmental biology · 2026Review
- Cell cycle dependent methylation of Dam1 contributes to kinetochore integrity and faithful chromosome segregation.PLoS genetics · 2025Article
- The Protein Response of Salt-TolerantJournal of fungi (Basel, Switzerland) · 2024Article
- Humanization reveals pervasive incompatibility of yeast and human kinetochore components.G3 (Bethesda, Md.) · 2023Article
- Misregulation of cell cycle-dependent methylation of budding yeast CENP-A contributes to chromosomal instability.Molecular biology of the cell · 2023Article
- Rio1 downregulates centromeric RNA levels to promote the timely assembly of structurally fit kinetochores.Nature communications · 2023Article
- Cdc48Ufd1/Npl4 segregase removes mislocalized centromeric histone H3 variant CENP-A from non-centromeric chromatin.Nucleic acids research · 2022Article
- Recent insights into mechanisms preventing ectopic centromere formation.Open biology · 2021Review
- R-loops at centromeric chromatin contribute to defects in kinetochore integrity and chromosomal instability in budding yeast.Molecular biology of the cell · 2021Article
- Conservation of centromeric histone 3 interaction partners in plants.Journal of experimental botany · 2020Article
- Skp, Cullin, F-box (SCF)-Met30 and SCF-Cdc4-Mediated Proteolysis of CENP-A Prevents Mislocalization of CENP-A for Chromosomal Stability in Budding Yeast.PLoS genetics · 2020Article
- Protein kinases in mitotic phosphorylation of budding yeast CENP-A.Current genetics · 2019Review
- Centromeric and ectopic assembly of CENP-A chromatin in health and cancer: old marks and new tracks.Nucleic acids research · 2019Review
- Conformational flexibility of histone variant CENP-AThe Journal of biological chemistry · 2018Article
- Heterochromatin and RNAi regulate centromeres by protecting CENP-A from ubiquitin-mediated degradation.PLoS genetics · 2018Article
- Chromatin assembly factor-1 (CAF-1) chaperone regulates Cse4 deposition into chromatin in budding yeast.Nucleic acids research · 2018Article
- Budding yeast CENP-ACell cycle (Georgetown, Tex.) · 2018Article
- Polo kinase Cdc5 associates with centromeres to facilitate the removal of centromeric cohesin during mitosis.Molecular biology of the cell · 2016Article
- Synergistic Control of Kinetochore Protein Levels by Psh1 and Ubr2.PLoS genetics · 2016Article
- Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Evolutionarily conserved histone H3 variant Cse4 and its homologues are essential components of specialized centromere (CEN)-specific nucleosomes and serve as an epigenetic mark for CEN identity and propagation. Cse4 is a critical determinant for the structure and function of the kinetochore and is required to ensure faithful chromosome segregation. The kinetochore protein Pat1 regulates the levels and spatial distribution of Cse4 at centromeres. Deletion of PAT1 results in altered structure of CEN chromatin and chromosome segregation errors. In this study, we show that Pat1 protects CEN-associated Cse4 from ubiquitination in order to maintain proper structure and function of the kinetochore in budding yeast. PAT1-deletion strains exhibit increased ubiquitination of Cse4 and faster turnover of Cse4 at kinetochores. Psh1, a Cse4-specific E3-ubiquitin ligase, interacts with Pat1 in vivo and contributes to the increased ubiquitination of Cse4 in pat1∆ strains. Consistent with a role of Psh1 in ubiquitination of Cse4, transient induction of PSH1 in a wild-type strain resulted in phenotypes similar to a pat1∆ strain, including a reduction in CEN-associated Cse4, increased Cse4 ubiquitination, defects in spatial distribution of Cse4 at kinetochores, and altered structure of CEN chromatin. Pat1 interacts with Scm3 and is required for its maintenance at kinetochores. In conclusion, our studies provide novel insights into mechanisms by which Pat1 affects the structure of CEN chromatin and protects Cse4 from Psh1-mediated ubiquitination for faithful chromosome segregation.
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