Evidence map›Paper›PMID 25833385›Full record

ArticleProtein & cell2015

Phylogenomics of non-model ciliates based on transcriptomic analyses.

Xiao Chen, Xiaolu Zhao, Xiaohui Liu, Alan Warren, Fangqing Zhao, Miao Miao

Open access · diamondAbstract read
In one paragraph

Article in Protein & cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 41 citations in OpenAlex.

  1. Review
  2. Article
  3. Genome content in the non-model ciliatebioRxiv : the preprint server for biology · 2024
    Article
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  15. The Genetic Diversity ofFrontiers in microbiology · 2016
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 2 countries.

Xiao Chen *College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049 China.
Xiaolu Zhao *Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao, 266003 China.
Xiaohui LiuCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049 China.
Alan WarrenDepartment of Life Sciences, Natural History Museum, Cromwell Road, London, SW7 5BD UK.
Fangqing ZhaoBeijing Institutes of Life Science, Chinese Academy of Sciences, Beijing, 100101 China.
Miao MiaoCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, 100049 China.
Ocean University of China · CNUniversity of Chinese Academy of Sciences · CNChinese Academy of Sciences · CNNatural History Museum · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ciliates are one of the oldest living eukaryotic unicellular organisms, widely distributed in the waters around the world. As a typical marine oligotrich ciliate, Strombidium sulcatum plays an important role in marine food webs and energy flow. Here we report the first deep sequencing and analyses of RNA-Seq data from Strombidium sulcatum. We generated 42,640 unigenes with an N50 of 1,451 bp after de novo assembly and removing rRNA, mitochondrial and bacteria contaminants. We employed SPOCS to detect orthologs from S. sulcatum and 17 other ciliates, and then carried out the phylogenomic reconstruction using 127 single copy orthologs. In phylogenomic analyses, concatenated trees have similar topological structures with concordance tree on the class level. Together with phylogenetic networks analysis, it aroused more doubts about the placement of Protocruzia, Mesodinium and Myrionecta. While epiplasmic proteins are known to be related to morphological characteristics, we found the potential relationship between gene expression of epiplasmic proteins and morphological characteristics. This work supports the use of high throughput approaches for phylogenomic analysis as well as correlation analysis between expression level of target genes and morphological characteristics.

Indexed as

CiliophoraPhylogenyRNA, ProtozoanTranscriptomeRNA, Protozoan

Identifiers

PMID25833385
PMCPMC4417680
OpenAlexW2062651196

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.