ArticleRNA biology2015
Four-leaf clover qRT-PCR: A convenient method for selective quantification of mature tRNA.
Article in RNA biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Updated guide to RNA quantification by RNA sequencing and reverse transcription-qPCR.Molecules and cells · 2026Article
- Tissue-specific role of dTrmO in threonine decoding during Drosophila melanogaster development.Biological research · 2026Article
- Quantification of tRNA mRNA (New York, N.Y.) · 2024Article
- Biallelic variants in GTF3C5, a regulator of RNA polymerase III-mediated transcription, cause a multisystem developmental disorder.Human genetics · 2024Article
- tRNA therapeutics for genetic diseases.Nature reviews. Drug discovery · 2024Review
- Structural-profiling of low molecular weight RNAs by nanopore trapping/translocation using Mycobacterium smegmatis porin A.Nature communications · 2021Article
- The human tRNA-guanine transglycosylase displays promiscuous nucleobase preference but strict tRNA specificity.Nucleic acids research · 2021Article
- tRNA-modifying enzyme mutations induce codon-specific mistranslation and protein aggregation in yeast.RNA biology · 2021Article
- Transcriptome-wide Mapping of Internal NMolecular cell · 2019Article
- RNA ligation of very small pseudo nick structures by T4 RNA ligase 2, leading to efficient production of versatile RNA rings.RSC advances · 2019Article
- Article
- Attenuated Codon Optimality Contributes to Neural-Specific mRNA Decay in Drosophila.Cell reports · 2018Article
- Deciphering the reading of the genetic code by near-cognate tRNA.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Protein folding and tRNA biology.Biophysical reviews · 2017Review
- YAMAT-seq: an efficient method for high-throughput sequencing of mature transfer RNAs.Nucleic acids research · 2017Article
- Genome-wide identification and characterization of tRNA-derived RNA fragments in land plants.Plant molecular biology · 2017Article
- Article
- Sex hormone-dependent tRNA halves enhance cell proliferation in breast and prostate cancers.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Transfer RNAs (tRNAs) play a central role in translation and also recently appear to have a variety of other functions in biological processes beyond translation. Here we report the development of Four-Leaf clover qRT-PCR (FL-PCR), a convenient PCR-based method, which can specifically quantify individual mature tRNA species. In FL-PCR, T4 RNA ligase 2 specifically ligates a stem-loop adapter to mature tRNAs but not to precursor tRNAs or tRNA fragments. Subsequent TaqMan qRT-PCR amplifies only unmodified regions of the tRNA-adapter ligation products; therefore, FL-PCR quantification is not influenced by tRNA post-transcriptional modifications. FL-PCR has broad applicability for the quantification of various tRNAs in different cell types, and thus provides a much-needed simple method for analyzing tRNA abundance and heterogeneity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.