ArticlePharmaceutical research2015
Intermolecular Interactions and the Viscosity of Highly Concentrated Monoclonal Antibody Solutions.
Article in Pharmaceutical research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Article
- Article
- A Review on Lymphatic Uptake of Large Therapeutic Proteins after Subcutaneous Injection.Pharmaceutical research · 2026Review
- Temperature and excipient mediated modulation of monoclonal antibody interactions revealed bymAbs · 2025Article
- Advanced nanotheranostic approaches for targeted glioblastoma treatment: a synergistic fusion of CRISPR-Cas gene editing, AI-driven tumor profiling, and BBB-modulation.Medical oncology (Northwood, London, England) · 2025Review
- Stability of Protein Pharmaceuticals: Recent Advances.Pharmaceutical research · 2024Review
- Utility of High Resolution 2D NMR Fingerprinting in Assessing Viscosity of Therapeutic Monoclonal Antibodies.Pharmaceutical research · 2022Article
- Discovery of compounds with viscosity-reducing effects on biopharmaceutical formulations with monoclonal antibodies.Computational and structural biotechnology journal · 2022Article
- Assessment of Antibody Self-Interaction by Bio-Layer-Interferometry as a Tool for Early Stage Formulation Development.Pharmaceutical research · 2020Article
- Net charge of antibody complementarity-determining regions is a key predictor of specificity.Protein engineering, design & selection : PEDS · 2018Article
- In Silico Prediction of Diffusion Interaction Parameter (kPharmaceutical research · 2018Article
- Viscosity Control of Protein Solution by Small Solutes: A Review.Current protein & peptide science · 2018Review
- Effects of Histidine and Sucrose on the Biophysical Properties of a Monoclonal Antibody.Pharmaceutical research · 2017Article
- A new approach to quantification of mAb aggregates using peptide affinity probes.Scientific reports · 2017Article
- Contrasting the Influence of Cationic Amino Acids on the Viscosity and Stability of a Highly Concentrated Monoclonal Antibody.Pharmaceutical research · 2017Article
- Review
- Article
- Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe large increase in viscosity of highly concentrated monoclonal antibody solutions can be challenging for downstream processing, drug formulation, and delivery steps. The objective of this work was to examine the viscosity of highly concentrated solutions of a high purity IgG1 monoclonal antibody over a wide range of protein concentrations, solution pH, ionic strength, and in the presence / absence of different excipients.
methodsExperiments were performed with an IgG1 monoclonal antibody provided by Amgen. The steady-state viscosity was evaluated using a Rheometrics strain-controlled rotational rheometer with a concentric cylinder geometry.
resultsThe viscosity data were well-described by the Mooney equation. The data were analyzed in terms of the antibody virial coefficients obtained from osmotic pressure data evaluated under the same conditions. The viscosity coefficient in the absence of excipients was well correlated with the third osmotic virial coefficient, which has a negative value (corresponding to short range attractive interactions) at the pH and ionic strength examined in this work.
conclusionsThese results provide important insights into the effects of intermolecular protein-protein interactions on the behavior of highly concentrated antibody solutions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.