ArticleScientific reports2015
A cost-effective RNA sequencing protocol for large-scale gene expression studies.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
60 citing papers in PubMed, 1 synthesis or guideline pooled it.
- RNA-seq research landscape in Africa: systematic review reveals disparities and opportunities.European journal of medical research · 2023Pooled it
- Enhancer Reprogramming Reveals the Tumorigenic Role of PTPRZ1 in Lung Squamous Cell Carcinoma.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Transcriptomic landscape around wound bed defines regenerative versus non-regenerative outcomes in mouse digit amputation.PLoS computational biology · 2025Article
- Review
- Optimal solution to the set cover problem with a vicinity constraint for estimating genotype tissue expression profiles.Bioinformatics advances · 2025Article
- Unlocking the Puzzle of Mammalian Transfection: The Role of the RNA-sensing-Mediated Interferon Response in the Cellular Defense Against Foreign DNA Intrusion.International journal of biological sciences · 2025Article
- Modulating the mesenchymal stromal cell microenvironment alters exosome RNA content and ligament healing capacity.Stem cells (Dayton, Ohio) · 2024Article
- The role of mitochondria in sex- and age-specific gene expression in a species without sex chromosomes.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Unraveling the complex relationship between mRNA and protein abundances: a machine learning-based approach for imputing protein levels from RNA-seq data.NAR genomics and bioinformatics · 2024Article
- The role of mitochondria in sex- and age-specific gene expression in a species without sex chromosomes.bioRxiv : the preprint server for biology · 2023Article
- Affordable, accurate and unbiased RNA sequencing by manual library miniaturization: A case study in barley.Plant biotechnology journal · 2023Article
- Transcriptomic clock predicts vascular changes of prodromal diabetic retinopathy.Scientific reports · 2023Article
- North African fox genomes show signatures of repeated introgression and adaptation to life in deserts.Nature ecology & evolution · 2023Article
- SOX18-enforced expression diverts hemogenic endothelium-derived progenitors from T towards NK lymphoid pathways.iScience · 2023Article
- HOXA3 functions as the on-off switch to regulate the development of hESC-derived third pharyngeal pouch endoderm through EPHB2-mediated Wnt pathway.Frontiers in immunology · 2023Article
- Bacterial Transcription Factors Bind to Coding Regions and Regulate Internal Cryptic Promoters.mBio · 2022Article
- A mechanism for red coloration in vertebrates.Current biology : CB · 2022Article
- RNA-seq and Mitochondrial DNA Analysis of Adrenal Gland Metastatic Tissue in a Patient with Renal Cell Carcinoma.Biology · 2022Article
- Recent developments and future directions in meta-analysis of differential gene expression in livestock RNA-Seq.Frontiers in genetics · 2022Review
- Data-driven network models for genetic circuits from time-series data with incomplete measurements.Journal of the Royal Society, Interface · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
RNA sequencing has increasingly become an indispensable tool for biological research. While sequencing costs have fallen dramatically in recent years, the current cost of RNA sequencing, nonetheless, remains a barrier to even more widespread adoption. Here, we present a simple RNA sequencing protocol with substantially reduced costs. This protocol uses as little as 10 ng of total RNA, allows multiplex sequencing of up to 96 samples per lane, and is strand specific. Extensive validation using human embryonic stem cells showed high consistency between technical replicates at various multiplexing levels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.