ArticleAutophagy2015
AU4S: a novel synthetic peptide to measure the activity of ATG4 in living cells.
Article in Autophagy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Acetylation dynamics of MrATG4 governing autophagy-mediated conidiation in an entomopathogenic fungus.PLoS pathogens · 2026Article
- Article
- TFEB SUMOylation in macrophages accelerates atherosclerosis by promoting the formation of foam cells through inhibiting lysosomal activity.Cellular and molecular life sciences : CMLS · 2023Article
- Role of ATG7-dependent non-autophagic pathway in angiogenesis.Frontiers in pharmacology · 2023Review
- Deacetylation of ATG4B promotes autophagy initiation under starvation.Science advances · 2022Article
- Autophagy responsive intra-intercellular delivery nanoparticles for effective deep solid tumor penetration.Journal of nanobiotechnology · 2022Article
- Response of Leucine-Rich Repeat Domain-Containing Protein in Haemaphysalis longicornis to Babesia microti Infection and Its Ligand Identification.Infection and immunity · 2021Article
- Overexpression of ATG4a promotes autophagy and proliferation, and inhibits apoptosis in lens epithelial cells via the AMPK and Akt pathways.Molecular medicine reports · 2020Article
- Targeting ATG4 in Cancer Therapy.Cancers · 2019Review
- Identification of Kinases and Phosphatases That Regulate ATG4B Activity by siRNA and Small Molecule Screening in Cells.Frontiers in cell and developmental biology · 2018Article
- Article
- AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells.Autophagy · 2018Article
- Autophagy-regulating protease Atg4: structure, function, regulation and inhibition.The Journal of antibiotics · 2017Review
- Measurement of the Activity of the Atg4 Cysteine Proteases.Methods in enzymology · 2017Review
- TRAIL DR5-CTSB crosstalk participates in breast cancer autophagy initiated by SAHA.Cell death discovery · 2017Article
- Dichloroacetate and metformin synergistically suppress the growth of ovarian cancer cells.Oncotarget · 2016Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ATG4 plays a key role in autophagy induction, but the methods for monitoring ATG4 activity in living cells are limited. Here we designed a novel fluorescent peptide named AU4S for noninvasive detection of ATG4 activity in living cells, which consists of the cell-penetrating peptide (CPP), ATG4-recognized sequence "GTFG," and the fluorophore FITC. Additionally, an ATG4-resistant peptide AG4R was used as a control. CPP can help AU4S or AG4R to penetrate cell membrane efficiently. AU4S but not AG4R can be recognized and cleaved by ATG4, leading to the change of fluorescence intensity. Therefore, the difference between AU4S- and AG4R-measured fluorescence values in the same sample, defined as "F-D value," can reflect ATG4 activity. By detecting the F-D values, we found that ATG4 activity paralleled LC3B-II levels in rapamycin-treated cells, but neither paralleled LC3B-II levels in starved cells nor presented a correlation with LC3B-II accumulation in WBCs from healthy donors or leukemia patients. However, when DTT was added to the system, ATG4 activity not only paralleled LC3B-II levels in starved cells in the presence or absence of autophagy inhibitors, but also presented a positive correlation with LC3B-II accumulation in WBCs from leukemia patients (R(2) = 0.5288). In conclusion, this study provides a convenient, rapid, and quantitative method to monitor ATG4 activity in living cells, which may be beneficial to basic and clinical research on autophagy.
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