ArticlePloS one2015
Antibiotic resistance modulation and modes of action of (-)-α-pinene in Campylobacter jejuni.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 130 citations in OpenAlex.
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- Bioactive Potential of Terpenes from Mediterranean Scrub Plants: A Review.Molecules (Basel, Switzerland) · 2025Review
- Profiling of Pharmaceutical Sludge Microbiome, Resistome and Secondary Metabolites Using Metagenomics.Indian journal of microbiology · 2025Article
- Oxidation of α-Pinene on the Ti-SBA-15 Catalyst Obtained Using Orange Peel Waste as Components of the Synthesis Gel.Molecules (Basel, Switzerland) · 2025Article
- Phytochemical Analysis and Pharmaceutical Applications of Monoterpenoids Present in the Essential Oil ofAdvances in pharmacological and pharmaceutical sciences · 2025Review
- Phytochemical Composition, Antibacterial Activity, Modes of Action, and Antibiotic Resistance-Modifying Effects ofScientifica · 2025Article
- Holistic monitoring of Campylobacter jejuni biofilms with NanoLuc bioluminescence.Applied microbiology and biotechnology · 2024Article
- Elucidation of the mechanisms of fluconazole resistance and repurposing treatment options against urinary Candida spp. isolated from hospitalized patients in Alexandria, Egypt.BMC microbiology · 2024Article
- Harnessing medicinal plant compounds for the control of Campylobacter in foods: a comprehensive review.Veterinary research communications · 2024Review
- The influence of drying and storage conditions on the volatilome and cannabinoid content of Cannabis sativa L. inflorescences.Analytical and bioanalytical chemistry · 2024Article
- Bacterial Efflux Pump Inhibitors Reduce Antibiotic Resistance.Pharmaceutics · 2024Review
- Characterization of Nanohybridosomes from Lipids and Spruce Homogenate Containing Extracellular Vesicles.International journal of nanomedicine · 2024Article
- Mixture design of α-pinene, α-terpineol, and 1,8-cineole: A multiobjective response followed by chemometric approaches to optimize the antibacterial effect against various bacteria and antioxidant activity.Food science & nutrition · 2024Article
- The Wild Carrot (Plants (Basel, Switzerland) · 2023Review
- The effects of alpha-pinene on the Nrf2-HO1 signaling pathway in gastric damage in rats.Molecular biology reports · 2023Article
- The comparative perspective of phytochemistry and biological properties of the Apiaceae family plants.Scientific reports · 2023Article
- Variation in the Chemical Composition of Endemic Specimens ofPlants (Basel, Switzerland) · 2023Article
- Article
- Tryptanthrin Reduces Campylobacter jejuni Colonization in the Chicken Gut by a Bactericidal Mechanism.Applied and environmental microbiology · 2023Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of the study was to investigate the mode of action of (-)-α-pinene in terms of its modulation of antibiotic resistance in Campylobacter jejuni. Broth microdilution and ethidium bromide accumulation assays were used to evaluate the (-)-α-pinene antimicrobial activity, modulation of antimicrobial resistance, and inhibition of antimicrobial efflux. The target antimicrobial efflux systems were identified using an insertion mutagenesis approach, and C. jejuni adaptation to (-)-α-pinene was evaluated using DNA microarrays. Knock-out mutants of the key up-regulated transcriptional regulators hspR and hrcA were constructed to investigate their roles in C. jejuni adaptation to several stress factors, including osmolytes, and pH, using Biolog phenotypical microarrays. Our data demonstrate that (-)-α-pinene efficiently modulates antibiotic resistance in C. jejuni by decreasing the minimum inhibitory concentrations of ciprofloxacin, erythromycin and triclosan by up to 512-fold. Furthermore, (-)-α-pinene promotes increased expression of cmeABC and another putative antimicrobial efflux gene, Cj1687. The ethidium bromide accumulation was greater in the wild-type strain than in the antimicrobial efflux mutant strains, which indicates that these antimicrobial efflux systems are a target of action of (-)-α-pinene. Additionally, (-)-α-pinene decreases membrane integrity, which suggests that enhanced microbial influx is a secondary mode of action of (-)-α-pinene. Transcriptomic analysis indicated that (-)-α-pinene disrupts multiple metabolic pathways, and particularly those involved in heat-shock responses. Thus, (-)-α-pinene has significant activity in the modulation of antibiotic resistance in C. jejuni, which appears to be mediated by multiple mechanisms that include inhibition of microbial efflux, decreased membrane integrity, and metabolic disruption. These data warrant further studies on (-)-α-pinene to develop its use in the control of antibiotic resistance in Campylobacter.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.