ArticlePloS one2015
Understanding of the viscoelastic response of the human corneal stroma induced by riboflavin/UV-a cross-linking at the nano level.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Multivariate Analysis of the Spatial Distance Between the Corneal Thinnest Point and Maximum Keratometry in Keratoconic Corneas: Association with Disease Severity.Diagnostics (Basel, Switzerland) · 2026Article
- The Science and Clinical Evolution of Corneal Cross-Linking: Mechanism of Action, Ultra-Structural Changes, Clinical Indications, and Emerging Treatment Strategies.Ophthalmology and therapy · 2026Review
- Global spatiotemporal biomechanics using video swin transformer: multiscale validation and clinical impact for keratoconus suspects.NPJ digital medicine · 2026Article
- Molecular and Biomechanical Changes in the Anterior Segment of High Myopic Eyes.Annals of biomedical engineering · 2026Review
- Nanoindentation under retrocorneal pressure to determine the biomechanical response of the human cornea to accelerated UVA crosslinking and riboflavin osmolarity ex vivo.Scientific reports · 2025Article
- Crosslinking of tarsal collagen as a hypothetical therapy for dry eye disease.Medical hypothesis, discovery & innovation ophthalmology journal · 2025Article
- Recent advances in medicinal compounds related to corneal crosslinking.Frontiers in pharmacology · 2023Review
- Micron-scale hysteresis measurement using dynamic optical coherence elastography.Biomedical optics express · 2022Article
- Silk Film Stiffness Modulates Corneal Epithelial Cell Mechanosignaling.Macromolecular chemistry and physics · 2021Article
- A review of corneal biomechanics: Mechanisms for measurement and the implications for refractive surgery.Indian journal of ophthalmology · 2020Review
- Assessment of the influence of viscoelasticity of cornea in animal ex vivo model using air-puff optical coherence tomography and corneal hysteresis.Journal of biophotonics · 2019Article
- Whether Keratectasia Area Shown in Corneal Topography Is Appropriate for Evaluating the Effect of Corneal Cross-Linking for Keratoconus: A 12-Month Follow-Up Study.BioMed research international · 2019Article
- Current perspectives on corneal collagen crosslinking (CXL).Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2018Review
- Transient viscous response of the human cornea probed with the Surface Force Apparatus.PloS one · 2018Article
- Optical coherence elastography in ophthalmology.Journal of biomedical optics · 2017Review
- Multiscale Investigation of the Depth-Dependent Mechanical Anisotropy of the Human Corneal Stroma.Investigative ophthalmology & visual science · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo investigate the viscoelastic changes of the human cornea induced by riboflavin/UV-A cross-linking using Atomic Force Microscopy (AFM) at the nano level.
methodsSeven eye bank donor corneas were investigated, after gently removing the epithelium, using a commercial AFM in the force spectroscopy mode. Silicon cantilevers with tip radius of 10 nm and spring elastic constants between 26- and 86-N/m were used to probe the viscoelastic properties of the anterior stroma up to 3 µm indentation depth. Five specimens were tested before and after riboflavin/UV-A cross-linking; the other two specimens were chemically cross-linked using glutaraldehyde 2.5% solution and used as controls. The Young's modulus (E) and the hysteresis (H) of the corneal stroma were quantified as a function of the application load and scan rate.
resultsThe Young's modulus increased by a mean of 1.1-1.5 times after riboflavin/UV-A cross-linking (P<0.05). A higher increase of E, by a mean of 1.5-2.6 times, was found in chemically cross-linked specimens using glutaraldehyde 2.5% (P<0.05). The hysteresis decreased, by a mean of 0.9-1.5 times, in all specimens after riboflavin/UV-A cross-linking (P<0.05). A substantial decrease of H, ranging between 2.6 and 3.5 times with respect to baseline values, was observed in glutaraldehyde-treated corneas (P<0.05).
conclusionsThe present study provides the first evidence that riboflavin/UV-A cross-linking induces changes of the viscoelastic properties of the cornea at the scale of stromal molecular interactions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.