Evidence map›Paper›PMID 25830356›Full record

ArticleScientific reports2015

Involvement of GluR2 up-regulation in neuroprotection by electroacupuncture pretreatment via cannabinoid CB1 receptor in mice.

Zhaoyu Liu, Xiyao Chen, Yang Gao, Sisi Sun, Lei Yang, Qianzi Yang, Fuhai Bai, Lize Xiong, Qiang Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed, 1 pooled it
11.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 1 synthesis or guideline pooled it, 48 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Zhaoyu LiuDepartment of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Xiyao ChenDepartment of Physiology, Fourth Military Medical University, Xi'an 710032, China.
Yang GaoDepartment of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Sisi SunDepartment of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Lei YangDepartment of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Qianzi YangDepartment of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Fuhai BaiDepartment of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Lize XiongDepartment of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Qiang WangDepartment of Anesthesiology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Air Force Medical University · CNXijing Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We investigated whether glutamate receptor subunit 2 (GluR2) is involved in EA pretreatment-induced neuroprotection via cannabinoid CB1 receptors (CB1R) after global cerebral ischemia in mice. Two hours after electric acupuncture (EA) pretreatment, global cerebral ischemia (GCI) was induced by bilateral common carotid artery occlusion (BCCAO) for 20 min. The GluR2 expression was examined in the hippocampus after reperfusion. Cell survival, neuronal apoptosis, the Bax/Bcl-2 ratio and neurological scores were evaluated at 24 h after BCCAO in the presence or absence of the GluR2 inhibitor. Furthermore, the GluR2 was determined in the presence and absence of CB1R inhibitor. Our results showed EA pretreatment enhanced expression of GluR2 in the hippocampus 2 h after reperfusion. Moreover, EA pretreatment improved neurological outcome, promoted cell survival, inhibited neuronal apoptosis, and decreased the Bax/Bcl-2 ratio after reperfusion. GluR2 knockdown by GluR2 siRNA effectively reversed the beneficial effects of EA pretreatment. Furthermore, CB1R siRNA and two CB1R antagonists blocked the elevation of GluR2 expression by EA pretreatment, whereas the two CB1R agonists up-regulated GluR2 expression as EA pretreatment. In conclusion, GluR2 up-regulation is involved in neuroprotection of EA pretreatment against GCI through CB1R, suggesting that GluR2 may be a novel target for stroke intervention.

Indexed as

ElectroacupunctureGene Expression RegulationAnimalsApoptosisArachidonic AcidsBrain IschemiaCell SurvivalDisease Models, AnimalDown-RegulationEndocannabinoidsGene Knockdown TechniquesGlyceridesHippocampusMicePyramidal CellsReceptor, Cannabinoid, CB1Arachidonic AcidsEndocannabinoidsglutamate receptor ionotropic, AMPA 2Glyceridesglyceryl 2-arachidonateReceptor, Cannabinoid, CB1Receptors, AMPA

Identifiers

PMID25830356
PMCPMC4381620
OpenAlexW1978206226

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.