ArticlePloS one2015
Selection of reference genes for transcriptional analysis of edible tubers of potato (Solanum tuberosum L.).
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Identification and validation of reference genes for quantitative real-time PCR in Corydalis saxicola under various abiotic stresses.Scientific reports · 2026Article
- Genome-wide identification and expression analysis of the SET domain-containing gene family in potato (Solanum tuberosum L.).BMC genomics · 2024Article
- StCoExpNet: a global co-expression network analysis facilitates identifying genes underlying agronomic traits in potatoes.Plant cell reports · 2024Article
- Global expression patterns ofFrontiers in plant science · 2023Article
- Selection and validation of reference genes for quantitative gene expression normalization in Taxus spp.Scientific reports · 2020Article
- Transcriptome-based identification and validation of reference genes for plant-bacteria interaction studies using Nicotiana benthamiana.Scientific reports · 2019Article
- Cowpea and abiotic stresses: identification of reference genes for transcriptional profiling by qPCR.Plant methods · 2018Article
- Article
- Selection and validation of reference genes for RT-qPCR analysis in potato under abiotic stress.Plant methods · 2017Article
- Evaluation of Sorghum [Sorghum bicolor (L.)] Reference Genes in Various Tissues and under Abiotic Stress Conditions for Quantitative Real-Time PCR Data Normalization.Frontiers in plant science · 2016Article
- Comparative Proteomics of Oxalate Downregulated Tomatoes Points toward Cross Talk of Signal Components and Metabolic Consequences during Post-harvest Storage.Frontiers in plant science · 2016Article
- Selection of Reference Genes for Expression Study in Pulp and Seeds of Theobroma grandiflorum (Willd. ex Spreng.) Schum.PloS one · 2016Article
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Authors and funding
8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Potato (Solanum tuberosum) yield has increased dramatically over the last 50 years and this has been achieved by a combination of improved agronomy and biotechnology efforts. Gene studies are taking place to improve new qualities and develop new cultivars. Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) is a bench-marking analytical tool for gene expression analysis, but its accuracy is highly dependent on a reliable normalization strategy of an invariant reference genes. For this reason, the goal of this work was to select and validate reference genes for transcriptional analysis of edible tubers of potato. To do so, RT-qPCR primers were designed for ten genes with relatively stable expression in potato tubers as observed in RNA-Seq experiments. Primers were designed across exon boundaries to avoid genomic DNA contamination. Differences were observed in the ranking of candidate genes identified by geNorm, NormFinder and BestKeeper algorithms. The ranks determined by geNorm and NormFinder were very similar and for all samples the most stable candidates were C2, exocyst complex component sec3 (SEC3) and ATCUL3/ATCUL3A/CUL3/CUL3A (CUL3A). According to BestKeeper, the importin alpha and ubiquitin-associated/ts-n genes were the most stable. Three genes were selected as reference genes for potato edible tubers in RT-qPCR studies. The first one, called C2, was selected in common by NormFinder and geNorm, the second one is SEC3, selected by NormFinder, and the third one is CUL3A, selected by geNorm. Appropriate reference genes identified in this work will help to improve the accuracy of gene expression quantification analyses by taking into account differences that may be observed in RNA quality or reverse transcription efficiency across the samples.
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