ArticlePloS one2015
Site-specific cassette exchange systems in the Aedes aegypti mosquito and the Plutella xylostella moth.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- ImprovedInternational journal of molecular sciences · 2023Article
- Mimicking superinfection exclusion disrupts alphavirus infection and transmission in the yellow fever mosquitoProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Optimizing CRE and PhiC31 mediated recombination inFrontiers in bioengineering and biotechnology · 2023Article
- Driving down malaria transmission with engineered gene drives.Frontiers in genetics · 2022Review
- EfficientFrontiers in genome editing · 2022Article
- Engineered action at a distance: Blood-meal-inducible paralysis in Aedes aegypti.PLoS neglected tropical diseases · 2019Article
- Modular cis-regulatory logic of yellow gene expression in silkmoth larvae.Insect molecular biology · 2019Article
- Molecular tools to create new strains for mosquito sexing and vector control.Parasites & vectors · 2018Review
- Molecular tools and genetic markers for the generation of transgenic sexing strains in Anopheline mosquitoes.Parasites & vectors · 2018Review
- Cre/lox-Recombinase-Mediated Cassette Exchange for Reversible Site-Specific Genomic Targeting of the Disease Vector, Aedes aegypti.Scientific reports · 2017Article
- Review
- Highly efficient and inducible DNA excision in transgenic silkworms using the FLP/FRT site-specific recombination system.Transgenic research · 2016Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetically engineered insects are being evaluated as potential tools to decrease the economic and public health burden of mosquitoes and agricultural pest insects. Here we describe a new tool for the reliable and targeted genome manipulation of pest insects for research and field release using recombinase mediated cassette exchange (RMCE) mechanisms. We successfully demonstrated the established ΦC31-RMCE method in the yellow fever mosquito, Aedes aegypti, which is the first report of RMCE in mosquitoes. A new variant of this RMCE system, called iRMCE, combines the ΦC31-att integration system and Cre or FLP-mediated excision to remove extraneous sequences introduced as part of the site-specific integration process. Complete iRMCE was achieved in two important insect pests, Aedes aegypti and the diamondback moth, Plutella xylostella, demonstrating the transferability of the system across a wide phylogenetic range of insect pests.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.