ArticleMolecular metabolism2015
TRAP-seq defines markers for novel populations of hypothalamic and brainstem LepRb neurons.
Article in Molecular metabolism, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 87 citations in OpenAlex.
- Pharmacokinetics and pharmacodynamics of mibavademab (a leptin receptor agonist): Results from a first-in-human phase I study.Clinical and translational science · 2024Trial
- Increased leptin signaling drives the response of hypothalamic LepRb neurons to diet-induced obesity.Molecular metabolism · 2026Article
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- Transcriptional regulation of ventral hippocampus-nucleus accumbens circuit excitability drives cocaine seeking.Science advances · 2026Article
- Neuroendocrine plasticity and crosstalk in pubertal development.Journal of neuroendocrinology · 2026Review
- A lateral hypothalamic neuronal population expressing leptin receptors counteracts anxiety to enable adaptive behavioral responses.Nature neuroscience · 2025Article
- In vivo profiling of the endothelium using 'AngioTag' zebrafish.Angiogenesis · 2025Article
- Molecular mechanisms and neural mediators of leptin action.Genes & development · 2025Review
- Age, sex, and cell type-resolved hypothalamic gene expression across the pubertal transition in mice.Biology of sex differences · 2024Article
- Article
- Review
- The Hypothalamic Epigenetic Landscape in Dietary Obesity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- 2022 Cannon lecture: an ode to signal transduction: how the growth hormone pathway revealed insight into height, malignancy, and obesity.American journal of physiology. Endocrinology and metabolism · 2023Review
- Suppression of food intake by Glp1r/Lepr-coexpressing neurons prevents obesity in mouse models.The Journal of clinical investigation · 2023Article
- l-Type amino acid transporter 1 in hypothalamic neurons in mice maintains energy and bone homeostasis.JCI insight · 2023Article
- Neither GLP-1 receptors nor GFRAL neurons are required for aversive or anorectic response to DON (vomitoxin).American journal of physiology. Regulatory, integrative and comparative physiology · 2023Article
- Estradiol regulates leptin sensitivity to control feeding via hypothalamic Cited1.Cell metabolism · 2023Article
- Effects of alcohol and PARP inhibition on RNA ribosomal engagement in cortical excitatory neurons.Frontiers in molecular neuroscience · 2023Article
- Molecular profile and response to energy deficit of leptin-receptor neurons in the lateral hypothalamus.Scientific reports · 2022Article
- Protocol to extract actively translated mRNAs from mouse hypothalamus by translating ribosome affinity purification.STAR protocols · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveLeptin acts via its receptor (LepRb) on multiple subpopulations of LepRb neurons in the brain, each of which controls specific aspects of energy balance. Despite the importance of LepRb-containing neurons, the transcriptome and molecular identity of many LepRb subpopulations remain undefined due to the difficulty of studying the small fraction of total cells represented by LepRb neurons in heterogeneous brain regions. Here we sought to examine the transcriptome of LepRb neurons directly and identify markers for functionally relevant LepRb subsets.
methodsWe isolated mRNA from mouse hypothalamic and brainstem LepRb cells by Translating Ribosome Affinity Purification (TRAP) and analyzed it by RNA-seq (TRAP-seq).
resultsTRAP mRNA from LepRb cells was enriched for markers of peptidergic neurons, while TRAP-depleted mRNA from non-LepRb cells was enriched for markers of glial and immune cells. Genes encoding secreted proteins that were enriched in hypothalamic and brainstem TRAP mRNA revealed subpopulations of LepRb neurons that contained neuropeptide-encoding genes (including prodynorphin, Pdyn) not previously used as functional markers for LepRb neurons. Furthermore, Pdyn (cre) -mediated ablation of Lepr (flox) in Pdyn-expressing neurons (LepRb (Pdyn) KO mice) blunted energy expenditure to promote obesity during high-fat feeding.
conclusionsTRAP-seq of CNS LepRb neurons defines the LepRb neuron transcriptome and reveals novel markers for previously unrecognized subpopulations of LepRb neurons.
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