Evidence map›Paper›PMID 25830018›Full record

ArticleGigaScience2015

Reconstructing a comprehensive transcriptome assembly of a white-pupal translocated strain of the pest fruit fly Bactrocera cucurbitae.

Sheina B Sim, Bernarda Calla, Brian Hall, Theodore DeRego, Scott M Geib

Abstract readDataset
In one paragraph

Article in GigaScience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Article
  2. Sex-basedGigaByte (Hong Kong, China) · 2022
    Article
  3. Article
  4. A Chromosome-Scale Assembly of theG3 (Bethesda, Md.) · 2017
    Article
  5. Article
  6. Article
  7. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Sheina B SimTropical Crop and Commodity Protection Research Unit, Daniel K. Inouye US Pacific Basin Agricultural Research Center, USDA Agricultural Research Services, Hilo, HI USA ; Department of Plant and Environmental Protection Sciences, University of Hawaii, Manoa, Honolulu, HI USA.
Bernarda CallaTropical Crop and Commodity Protection Research Unit, Daniel K. Inouye US Pacific Basin Agricultural Research Center, USDA Agricultural Research Services, Hilo, HI USA.
Brian HallTropical Crop and Commodity Protection Research Unit, Daniel K. Inouye US Pacific Basin Agricultural Research Center, USDA Agricultural Research Services, Hilo, HI USA ; Department of Plant and Environmental Protection Sciences, University of Hawaii, Manoa, Honolulu, HI USA.
Theodore DeRegoTropical Crop and Commodity Protection Research Unit, Daniel K. Inouye US Pacific Basin Agricultural Research Center, USDA Agricultural Research Services, Hilo, HI USA.
Scott M GeibTropical Crop and Commodity Protection Research Unit, Daniel K. Inouye US Pacific Basin Agricultural Research Center, USDA Agricultural Research Services, Hilo, HI USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBactrocera cucurbitae is a serious global agricultural pest. Basic genomic information is lacking for this species, and this would be useful to inform methods of control, damage mitigation, and eradication efforts. Here, we have sequenced, assembled, and annotated a comprehensive transcriptome for a mass-rearing sexing strain of this species. This forms a foundational genomic and transcriptomic resource that can be used to better understand the physiology and biochemistry of this insect as well as being a useful tool for population genetics.

findingsA transcriptome assembly was constructed containing 17,654 transcript isoforms derived from 10,425 unigenes. This transcriptome size is similar to reports from other Tephritid species and probably includes about 70-80% of the protein-coding genes in the genome. The dataset is publicly available in NCBI and GigaDB as a resource for researchers.

conclusionsFoundational knowledge on the protein-coding genes in B. cucurbitae will lead to improved resources for this species. Through comparison with a model system such as Drosophila as well as a growing number of related Tephritid transcriptomes, improved strategies can be developed to control this pest.

Indexed as

TranscriptomeAnimalsData CurationGenetics, PopulationPupaSequence Analysis, RNATephritidaeBactrocera cucurbitaeMelon flyRNA-SeqSITSterile insect techniqueTephritidaeTranslocationWhite-pupae

Identifiers

PMID25830018
PMCPMC4379760

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.