ArticleThe Plant cell2015
pH Regulation by NHX-Type Antiporters Is Required for Receptor-Mediated Protein Trafficking to the Vacuole in Arabidopsis.
Article in The Plant cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 135 citations in OpenAlex.
- TurboID-based proteomic profiling reveals proxitome of the IRT1 metal transporter and new insight into IRT1 endocytosis.The New phytologist · 2026Article
- Molecular mechanisms and evolutionary adaptations of transporters for photosynthates and specialized metabolites in plants.Plant cell reports · 2026Review
- Post-Golgi Trafficking in Plant Cells.Sub-cellular biochemistry · 2026Review
- Article
- Lithium in plants.The New phytologist · 2025Review
- Increased Apigenin in DNA-Edited Hexaploid Wheat Promoted Soil Bacterial Nitrogen Fixation and Improved Grain Yield Under Limiting Nitrogen Fertiliser.Plant biotechnology journal · 2025Article
- Structural and Functional Characterization of the Vacuolar-Type NaBiomolecules · 2025Article
- Detection of Cytosolic Ion Concentrations by the Trans-Golgi Network/Early Endosome is Important for Salt Tolerance.bioRxiv : the preprint server for biology · 2025Article
- Genome-Wide Identification of theInternational journal of molecular sciences · 2025Article
- A KRice (New York, N.Y.) · 2025Article
- Molecular tipping points in plant cell surface HQuantitative plant biology · 2025Review
- Salinity survival: molecular mechanisms and adaptive strategies in plants.Frontiers in plant science · 2025Review
- The vacuolar KScience advances · 2024Article
- Functional implication of the homotrimeric multidomain vacuolar sorting receptor 1 (VSR1) from Arabidopsis thaliana.Scientific reports · 2024Article
- Structure-Guided Identification of Critical Residues in the Vacuolar Cation/Proton Antiporter NHX1 fromPlants (Basel, Switzerland) · 2023Article
- Transcription Factor SiDi19-3 Enhances Salt Tolerance of Foxtail Millet andInternational journal of molecular sciences · 2023Article
- STOP1 and STOP1-like proteins, key transcription factors to cope with acid soil syndrome.Frontiers in plant science · 2023Review
- The vacuolar H+/Ca transporter CAX1 participates in submergence and anoxia stress responses.Plant physiology · 2022Article
- Sodium Accumulation in Infected Cells and Ion Transporters Mistargeting in Nodules ofInternational journal of molecular sciences · 2022Article
- Plant ESCRT protein ALIX coordinates with retromer complex in regulating receptor-mediated sorting of soluble vacuolar proteins.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein trafficking requires proper ion and pH homeostasis of the endomembrane system. The NHX-type Na(+)/H(+) antiporters NHX5 and NHX6 localize to the Golgi, trans-Golgi network, and prevacuolar compartments and are required for growth and trafficking to the vacuole. In the nhx5 nhx6 T-DNA insertional knockouts, the precursors of the 2S albumin and 12S globulin storage proteins accumulated and were missorted to the apoplast. Immunoelectron microscopy revealed the presence of vesicle clusters containing storage protein precursors and vacuolar sorting receptors (VSRs). Isolation and identification of complexes of VSRs with unprocessed 12S globulin by 2D blue-native PAGE/SDS-PAGE indicated that the nhx5 nhx6 knockouts showed compromised receptor-cargo association. In vivo interaction studies using bimolecular fluorescence complementation between VSR2;1, aleurain, and 12S globulin suggested that nhx5 nhx6 knockouts showed a significant reduction of VSR binding to both cargoes. In vivo pH measurements indicated that the lumens of VSR compartments containing aleurain, as well as the trans-Golgi network and prevacuolar compartments, were significantly more acidic in nhx5 nhx6 knockouts. This work demonstrates the importance of NHX5 and NHX6 in maintaining endomembrane luminal pH and supports the notion that proper vacuolar trafficking and proteolytic processing of storage proteins require endomembrane pH homeostasis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.