ArticleJournal of biomolecular NMR2015
Dynamic nuclear polarization of membrane proteins: covalently bound spin-labels at protein-protein interfaces.
Article in Journal of biomolecular NMR, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 62 citations in OpenAlex.
- DNP enhanced solid-state NMR of lattice-like microcrystalline protein assemblies facilitated by co-assembly with dinitroxide-tagged proteins.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2026Article
- Probing Biomolecular Interactions with Paramagnetic Nuclear Magnetic Resonance Spectroscopy.Chembiochem : a European journal of chemical biology · 2025Review
- TOAC spin-labeled peptides tailored for DNP-NMR studies in lipid membrane environments.Biophysical journal · 2021Article
- Targeted DNP for biomolecular solid-state NMR.Chemical science · 2021Review
- Competing Transfer Pathways in Direct and Indirect Dynamic Nuclear Polarization MAS NMR Experiments on HIV-1 Capsid Assemblies: Implications for Sensitivity and Resolution.Magnetic resonance (Gottingen, Germany) · 2021Article
- Dynamic Nuclear Polarization of Biomembrane Assemblies.Biomolecules · 2020Article
- In Situ Detection of Endogenous HIV Activation by Dynamic Nuclear Polarization NMR and Flow Cytometry.International journal of molecular sciences · 2020Article
- Biomolecular complex viewed by dynamic nuclear polarization solid-state NMR spectroscopy.Biochemical Society transactions · 2020Review
- A biradical-tagged phospholipid as a polarizing agent for solid-state MAS Dynamic Nuclear Polarization NMR of membrane proteins.Solid state nuclear magnetic resonance · 2019Article
- Electron spin density matching for cross-effect dynamic nuclear polarization.Chemical communications (Cambridge, England) · 2019Article
- Article
- New NMR tools for protein structure and function: Spin tags for dynamic nuclear polarization solid state NMR.Archives of biochemistry and biophysics · 2017Review
- Gd(iii) and Mn(ii) complexes for dynamic nuclear polarization: small molecular chelate polarizing agents and applications with site-directed spin labeling of proteins.Physical chemistry chemical physics : PCCP · 2016Article
- Molecular Rationale for Improved Dynamic Nuclear Polarization of Biomembranes.The journal of physical chemistry. B · 2016Article
- Efficient DNP NMR of membrane proteins: sample preparation protocols, sensitivity, and radical location.Journal of biomolecular NMR · 2016Article
- Low-temperature dynamic nuclear polarization with helium-cooled samples and nitrogen-driven magic-angle spinning.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2016Article
- Frequency swept microwaves for hyperfine decoupling and time domain dynamic nuclear polarization.Solid state nuclear magnetic resonance · 2015Article
- Dynamic nuclear polarization of nucleic acid with endogenously bound manganese.Journal of biomolecular NMR · 2015Article
- NMR studies of membrane proteins.Journal of biomolecular NMR · 2015Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
We demonstrate that dynamic nuclear polarization of membrane proteins in lipid bilayers may be achieved using a novel polarizing agent: pairs of spin labels covalently bound to a protein of interest interacting at an intermolecular interaction surface. For gramicidin A, nitroxide tags attached to the N-terminal intermolecular interface region become proximal only when bimolecular channels forms in the membrane. We obtained signal enhancements of sixfold for the dimeric protein. The enhancement effect was comparable to that of a doubly tagged sample of gramicidin C, with intramolecular spin pairs. This approach could be a powerful and selective means for signal enhancement in membrane proteins, and for recognizing intermolecular interfaces.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.