ArticleMethods in molecular biology (Clifton, N.J.)2015
Preparation of mononucleosomal templates for analysis of transcription with RNA polymerase using spFRET.
Article in Methods in molecular biology (Clifton, N.J.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- H2A.Z and H3:K56Q Affect Transcription Through Chromatin and Yeast FACT-Dependent Nucleosome Unfolding.International journal of molecular sciences · 2025Article
- Article
- Poly(ADP-ribose)polymerase 2 is zinc-dependent enzyme and nucleosome reorganizer.Cellular and molecular life sciences : CMLS · 2025Article
- Resveratrol Inhibits Nucleosome Binding and Catalytic Activity of PARP1.Biomolecules · 2024Article
- Interactions of Nucleosomes with Acidic Patch-Binding Peptides: A Combined Structural Bioinformatics, Molecular Modeling, Fluorescence Polarization, and Single-Molecule FRET Study.International journal of molecular sciences · 2023Article
- Epigallocatechin Gallate Affects the Structure of Chromatosomes, Nucleosomes and Their Complexes with PARP1.International journal of molecular sciences · 2023Article
- Structure and Dynamics of Compact Dinucleosomes: Analysis by Electron Microscopy and spFRET.International journal of molecular sciences · 2023Article
- Article
- N-Terminal Tails of Histones H2A and H2B Differentially Affect Transcription by RNA Polymerase II In Vitro.Cells · 2022Article
- NaMicroscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2022Article
- Electron microscopy analysis of ATP-independent nucleosome unfolding by FACT.Communications biology · 2022Article
- Mechanism of curaxin-dependent nucleosome unfolding by FACT.Frontiers in molecular biosciences · 2022Article
- Molecular Mechanisms of PARP-1 Inhibitor 7-Methylguanine.International journal of molecular sciences · 2020Article
- Time-resolved analysis of transcription through chromatin.Methods (San Diego, Calif.) · 2019Article
- Stabilization of Nucleosomes by Histone Tails and by FACT Revealed by spFRET Microscopy.Cancers · 2017Article
- Unfolding of core nucleosomes by PARP-1 revealed by spFRET microscopy.AIMS genetics · 2017Article
- Large-scale ATP-independent nucleosome unfolding by a histone chaperone.Nature structural & molecular biology · 2016Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Single positioned nucleosomes have been extensively employed as simple model experimental systems for analysis of various intranuclear processes. Here we describe an experimental system containing positioned mononucleosomes allowing transcription by various RNA polymerases. Each DNA template contains a pair of fluorescent labels (Cy3 and Cy5) allowing measuring relative distances between the neighboring coils of nucleosomal DNA using Forster resonance energy transfer (FRET). The single-particle FRET (spFRET) approach for analysis of DNA uncoiling from the histone octamer during transcription through chromatin is described in detail.
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