Evidence map›Paper›PMID 25826656›Full record

ArticleRNA biology2015

ASH1 mRNP-core factors form stable complexes in absence of cargo RNA at physiological conditions.

Franziska T Edelmann, Annika Niedner, Dierk Niessing

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RNA biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Franziska T Edelmanna Institute of Structural Biology ; Helmholtz Zentrum München - German Center for Environmental Health ; Neuherberg , Germany.
Annika Niedner
Dierk Niessing
Helmholtz Zentrum München · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Asymmetric ASH1 mRNA transport during mitosis of budding yeast constitutes one of the best-studied examples of mRNA localization. Recently, 2 studies used in vitro motility assays to prove that motile ASH1 mRNA-transport complexes can be reconstituted entirely from recombinant factors. Both studies, however, differed in their conclusions on whether cargo RNA itself is required for particle assembly and thus activation of directional transport. Here we provide direct evidence that stable complexes do assemble in absence of RNA at physiologic conditions and even at ionic strengths above cellular levels. These results directly confirm the previous notion that the ASH1 transport machinery is not activated by the cargo RNA itself, but rather through protein-protein interactions.

Indexed as

Gene Expression Regulation, FungalEscherichia coliMitosisMyosin Heavy ChainsMyosin Type VOsmolar ConcentrationPhosphorylationProtein MultimerizationRecombinant Fusion ProteinsRepressor ProteinsRibonucleoproteinsRNA-Binding ProteinsRNA, FungalRNA, MessengerRNA TransportSaccharomyces cerevisiaeASH1 protein, S cerevisiaemessenger ribonucleoproteinMyo4 protein, S cerevisiaeMyosin Heavy ChainsMyosin Type VRecombinant Fusion ProteinsRepressor ProteinsRibonucleoproteinsRNA-Binding ProteinsRNA, FungalRNA, MessengerSaccharomyces cerevisiae ProteinsSHE2 protein, S cerevisiaeSHE3 protein, S cerevisiaeASH1 mRNAbudding yeastin vitro reconstitutionmacromolecular assemblymRNPMyo4pmyosinRNA localizationShe2pShe3p

Identifiers

PMID25826656
PMCPMC4615642
OpenAlexW1874626961

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.