Evidence map›Paper›PMID 25826279›Full record

ArticlePloS one2015

Brain isoform glycogen phosphorylase as a novel hepatic progenitor cell marker.

Yu-Wen Huang, Chien-Chang Chiu, Ja-Der Liang, Ling-Ling Chiou, Guan-Tarn Huang, Ming-Jiun Yu, Hsuan-Shu Lee

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed, 1 pooled it
–field-weighted citation impact, top 95% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 synthesis or guideline pooled it, 2 citations in OpenAlex.

  1. Pooled it
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Yu-Wen HuangInstitute of Biotechnology, National Taiwan University, Taipei, Taiwan; Division of Medical Devices and Cosmetics, Food and Drug Administration, Ministry of Health and Welfare, Taipei, Taiwan.
Chien-Chang ChiuInstitute of Biotechnology, National Taiwan University, Taipei, Taiwan.
Ja-Der LiangDepartment of Internal Medicine, National Taiwan University, Taipei, Taiwan.
Ling-Ling ChiouLiver Disease Prevention and Treatment Research Foundation, Taipei, Taiwan.
Guan-Tarn HuangDepartment of Internal Medicine, National Taiwan University, Taipei, Taiwan.
Ming-Jiun YuInstitute of Biochemistry and Molecular Biology, National Taiwan University College of Medicine, Taipei, Taiwan.
Hsuan-Shu LeeInstitute of Biotechnology, National Taiwan University, Taipei, Taiwan; Department of Internal Medicine, National Taiwan University, Taipei, Taiwan; Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan.
National Taiwan University · TWAgricultural Biotechnology Research Center, Academia Sinica · TWMinistry of Health and Welfare · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

An appropriate liver-specific progenitor cell marker is a stepping stone in liver regenerative medicine. Here, we report brain isoform glycogen phosphorylase (GPBB) as a novel liver progenitor cell marker. GPBB was identified in a protein complex precipitated by a monoclonal antibody Ligab generated from a rat liver progenitor cell line Lig-8. Immunoblotting results show that GPBB was expressed in two liver progenitor cell lines Lig-8 and WB-F344. The levels of GPBB expression decreased in the WB-F344 cells under sodium butyrate (SB)-induced cell differentiation, consistent with roles of GPBB as a liver progenitor cell marker. Short hairpin RNA (shRNA)-mediated GPBB knockdown followed by glucose deprivation test shows that GPBB aids in liver progenitor cell survival under low glucose conditions. Furthermore, shRNA-mediated GPBB knockdown followed by SB-induced cell differentiation shows that reducing GPBB expression delayed liver progenitor cell differentiation. We conclude that GPBB is a novel liver progenitor cell marker, which facilitates liver progenitor cell survival under low glucose conditions and cell differentiation.

Indexed as

AnimalsButyric AcidCell DifferentiationCell LineCells, CulturedGene Knockdown TechniquesGlycogen PhosphorylaseGlycogen Phosphorylase, Brain FormImmunoprecipitationLiverRatsRats, Inbred F344Stem CellsButyric AcidGlycogen PhosphorylaseGlycogen Phosphorylase, Brain Form

Identifiers

PMID25826279
PMCPMC4380311
OpenAlexW2027930763

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.