ArticleProceedings of the National Academy of Sciences of the United States of America2015
Juvenile hormone-activated phospholipase C pathway enhances transcriptional activation by the methoprene-tolerant protein.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 94 citations in OpenAlex.
- Advances in the study of juvenile hormone signaling.Frontiers in physiology · 2026Pooled it
- Renaming the 'OS-D/CSP' Family (Part 2): '4-Cysteine Soluble Proteins' (4CSPs)-Intracellular Functions.Insects · 2026Review
- Characterization of the Crustacean Methyl Farnesoate Transcriptional Signaling Genes.International journal of molecular sciences · 2026Article
- MET goes to the membrane-A new place for the "old" juvenile hormone receptor.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Insect Flight and Lipid Metabolism: Beyond the Classic Knowledge.Advances in experimental medicine and biology · 2026Review
- Involvement of Taiman in juvenile hormone signaling controlling sexual maturation in a male moth.Current research in insect science · 2026Article
- The inhibition mechanism ofFrontiers in microbiology · 2026Review
- Juvenile hormone induces phosphorylation of insulin/insulin-like growth factor signaling proteins in previtellogenic Aedes aegypti mosquitoes.Insect science · 2025Article
- Membrane-localized MET engages PVR to mediate extranuclear juvenile hormone signaling inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Article
- The GPCR A35 regulates fecundity ofFrontiers in insect science · 2025Article
- Methoprene-Tolerant (Met) Acts as Methyl Farnesoate Receptor to Regulate Larva Metamorphosis in Mud Crab,International journal of molecular sciences · 2024Article
- Juvenile hormone signaling is indispensable for late embryogenesis in ametabolous and hemimetabolous insects.BMC biology · 2024Article
- Cyp6g2 is the major P450 epoxidase responsible for juvenile hormone biosynthesis in Drosophila melanogaster.BMC biology · 2024Article
- Juvenile hormones direct primordial germ cell migration to the embryonic gonad.Current biology : CB · 2024Article
- Juvenile hormone receptor Methoprene tolerant: Functions and applications.Vitamins and hormones · 2023Article
- Impact of JH Signaling on Reproductive Physiology of the Classical Insect Model,International journal of molecular sciences · 2022Article
- Juvenile hormone promotes paracellular transport of yolk proteins via remodeling zonula adherens at tricellular junctions in the follicular epithelium.PLoS genetics · 2022Article
- Inducible Expression of SeveralInsects · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Juvenile hormone (JH) is a key regulator of a wide diversity of developmental and physiological events in insects. Although the intracellular JH receptor methoprene-tolerant protein (MET) functions in the nucleus as a transcriptional activator for specific JH-regulated genes, some JH responses are mediated by signaling pathways that are initiated by proteins associated with plasma membrane. It is unknown whether the JH-regulated gene expression depends on the membrane-mediated signal transduction. In Aedes aegypti mosquitoes, we found that JH activated the phospholipase C (PLC) pathway and quickly increased the levels of inositol 1,4,5-trisphosphate, diacylglycerol, and intracellular calcium, leading to activation and autophosphorylation of calcium/calmodulin-dependent protein kinase II (CaMKII). When abdomens from newly emerged mosquitoes were cultured in vitro, the JH-activated gene expression was repressed substantially if specific inhibitors of PLC or CaMKII were added to the medium together with JH. In newly emerged female mosquitoes, RNAi-mediated depletion of PLC or CaMKII considerably reduced the expression of JH-responsive genes, including the Krüppel homolog 1 gene (AaKr-h1) and the early trypsin gene (AaET). JH-induced loading of MET to the promoters of AaKr-h1 and AaET was weakened drastically when either PLC or CaMKII was inactivated in the cultured tissues. Therefore, the results suggest that the membrane-initiated signaling pathway modifies the DNA-binding activity of MET via phosphorylation and thus facilitates the genomic responses to JH. In summary, this study reveals an interplay of genomic and nongenomic signaling mechanisms of JH.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.