ArticleProceedings of the National Academy of Sciences of the United States of America2015
Sphingomyelin distribution in lipid rafts of artificial monolayer membranes visualized by Raman microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 138 citations in OpenAlex.
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- Advanced multimodal imaging: FLIM, PLIM, and FluoRaman enabled by novel diarylacetylene probes.The Analyst · 2026Article
- High-throughput spontaneous Raman imaging of biological specimens with water background reduction.Biomedical optics express · 2026Article
- Probing Rigidity and Fluidity in the Interfacial Region of Lipid Bilayers with a Novel IR Probe.The journal of physical chemistry. B · 2025Article
- Role of Redox Homeostasis in the Communication Between Brain and Liver Through Extracellular Vesicles.Antioxidants (Basel, Switzerland) · 2024Review
- Extracting the Heterogeneous 3D Structure of Molecular Films Using Higher Dimensional SFG Microscopy.The journal of physical chemistry letters · 2024Article
- Polar Glycerolipids and Membrane Lipid Rafts.International journal of molecular sciences · 2024Review
- Cholesterol and Lipid Rafts in the Biogenesis of Amyloid-β Protein and Alzheimer's Disease.Annual review of biophysics · 2024Review
- Assessment of Drug Activities againstPathogens (Basel, Switzerland) · 2024Article
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- Biomedical applications, perspectives and tag design concepts in the cell - silent Raman window.RSC chemical biology · 2024Review
- A Raman spectral marker for the iso-octyl chain structure of cholesterol.Analytical science advances · 2024Article
- Proactive and reactive roles of TGF-β in cancer.Seminars in cancer biology · 2023Review
- Eukaryotic Cell Membranes: Structure, Composition, Research Methods and Computational Modelling.International journal of molecular sciences · 2023Review
- Raman Spectroscopy Characterization of Multi-Functionalized Liposomes as Drug-Delivery Systems for Neurological Disorders.Nanomaterials (Basel, Switzerland) · 2023Article
- Raman Spectroscopy as a Tool to Study the Pathophysiology of Brain Diseases.International journal of molecular sciences · 2023Review
- Treatment of tubular damage in high-fat-diet-fed obese mice using sodium-glucose co-transporter inhibitors.PloS one · 2023Article
- Raman Spectroscopy for Chemical Biology Research.Journal of the American Chemical Society · 2022Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sphingomyelin (SM) and cholesterol (chol)-rich domains in cell membranes, called lipid rafts, are thought to have important biological functions related to membrane signaling and protein trafficking. To visualize the distribution of SM in lipid rafts by means of Raman microscopy, we designed and synthesized an SM analog tagged with a Raman-active diyne moiety (diyne-SM). Diyne-SM showed a strong peak in a Raman silent region that is free of interference from intrinsic vibrational modes of lipids and did not appear to alter the properties of SM-containing monolayers. Therefore, we used Raman microscopy to directly visualize the distribution of diyne-SM in raft-mimicking domains formed in SM/dioleoylphosphatidylcholine/chol ternary monolayers. Raman images visualized a heterogeneous distribution of diyne-SM, which showed marked variation, even within a single ordered domain. Specifically, diyne-SM was enriched in the central area of raft domains compared with the peripheral area. These results seem incompatible with the generally accepted raft model, in which the raft and nonraft phases show a clear biphasic separation. One of the possible reasons is that gradual changes of SM concentration occur between SM-rich and -poor regions to minimize hydrophobic mismatch. We believe that our technique of hyperspectral Raman imaging of a single lipid monolayer opens the door to quantitative analysis of lipid membranes by providing both chemical information and spatial distribution with high (diffraction-limited) spatial resolution.
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