ArticleProceedings of the National Academy of Sciences of the United States of America2015
The circadian oscillator in Synechococcus elongatus controls metabolite partitioning during diurnal growth.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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Who cites it
85 citing papers in PubMed, 144 citations in OpenAlex.
- Circadian temperature compensation is intrinsically linked with metabolism and redox signaling.Nature communications · 2026Article
- Distinct Lipid Compositions in Seawater and the Sea Surface Microlayer Measured with Liquid Chromatography and High-Resolution Mass Spectrometry.ACS earth & space chemistry · 2026Article
- KaiC-dependent circadian positioning of RNA polymerase and ribosome in cyanobacteria.iScience · 2026Article
- Rapid Adaptation of Cyanobacteria to Environmental Perturbations Is Achieved Through Structural Remodeling of the Proteome.Molecular & cellular proteomics : MCP · 2026Article
- RpaA Overexpression Enhances Bioluminescence Intensity and Elevates Rhythmic Extracellular Vesicle Yield inLife (Basel, Switzerland) · 2026Article
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- Systematic review on proliferation of microalgae and carbon sequestration potential for sustainability.World journal of microbiology & biotechnology · 2026Article
- Gut microbiota circadian rhythms: a key regulator of immunometabolic homeostasis.Acta biochimica et biophysica Sinica · 2026Review
- Optimising environmental factors for maximal lactate productivity in Synechocystis sp. PCC 6803 through a design of experiments approach.Biotechnology for biofuels and bioproducts · 2025Article
- Diurnal rhythm causes metabolic crises in the cyanobacterial mutants of c-di-AMP signaling cascade.iScience · 2025Article
- Mutations in the circadian cycle drive adaptive plasticity in cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Plastoquinone redox status influences carboxysome integrity via a RpaA- and reactive oxygen species-dependent regulatory network.The Plant journal : for cell and molecular biology · 2025Article
- Composition and rhythmic variations in the microbiome of Southwestern Atlantic corals.Environmental microbiome · 2025Article
- Self-sustained rhythmic behavior ofPNAS nexus · 2025Article
- Environmental and molecular noise buffering by the cyanobacterial clock in individual cells.Nature communications · 2025Article
- Cyanobacterial circadian regulation enhances bioproduction under subjective nighttime through rewiring of carbon partitioning dynamics, redox balance orchestration, and cell cycle modulation.Microbial cell factories · 2025Article
- Model cyanobacterial consortia reveal a consistent core microbiome independent of inoculation source or cyanobacterial host species.The ISME journal · 2025Article
- Two KaiABC systems control circadian oscillations in one cyanobacterium.Nature communications · 2024Article
- Endogenous clock-mediated regulation of intracellular oxygen dynamics is essential for diazotrophic growth of unicellular cyanobacteria.Nature communications · 2024Article
25 more citing papers are in PubMed but not listed here.
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4 authors at 1 institution in 1 country.
Funding
Abstract
Synechococcus elongatus PCC 7942 is a genetically tractable model cyanobacterium that has been engineered to produce industrially relevant biomolecules and is the best-studied model for a prokaryotic circadian clock. However, the organism is commonly grown in continuous light in the laboratory, and data on metabolic processes under diurnal conditions are lacking. Moreover, the influence of the circadian clock on diurnal metabolism has been investigated only briefly. Here, we demonstrate that the circadian oscillator influences rhythms of metabolism during diurnal growth, even though light-dark cycles can drive metabolic rhythms independently. Moreover, the phenotype associated with loss of the core oscillator protein, KaiC, is distinct from that caused by absence of the circadian output transcriptional regulator, RpaA (regulator of phycobilisome-associated A). Although RpaA activity is important for carbon degradation at night, KaiC is dispensable for those processes. Untargeted metabolomics analysis and glycogen kinetics suggest that functional KaiC is important for metabolite partitioning in the morning. Additionally, output from the oscillator functions to inhibit RpaA activity in the morning, and kaiC-null strains expressing a mutant KaiC phosphomimetic, KaiC-pST, in which the oscillator is locked in the most active output state, phenocopies a ΔrpaA strain. Inhibition of RpaA by the oscillator in the morning suppresses metabolic processes that normally are active at night, and kaiC-null strains show indications of oxidative pentose phosphate pathway activation as well as increased abundance of primary metabolites. Inhibitory clock output may serve to allow secondary metabolite biosynthesis in the morning, and some metabolites resulting from these processes may feed back to reinforce clock timing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.