ArticleJournal of bacteriology2015
Role of Bacillus subtilis DNA Glycosylase MutM in Counteracting Oxidatively Induced DNA Damage and in Stationary-Phase-Associated Mutagenesis.
Article in Journal of bacteriology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 24 citations in OpenAlex.
- Rapid Adaptation of Cyanobacteria to Environmental Perturbations Is Achieved Through Structural Remodeling of the Proteome.Molecular & cellular proteomics : MCP · 2026Article
- Characterization and Transcriptomic Analysis of AntarcticJournal of microbiology and biotechnology · 2026Article
- Live tracking of replisomes reveals nutrient-dependent regulation of replication elongation rates in Caulobacter crescentus.Current biology : CB · 2025Article
- Review
- 8-OxoG-Dependent Regulation of Global Protein Responses Leads to Mutagenesis and Stress Survival inAntioxidants (Basel, Switzerland) · 2024Article
- CRISPR/Cas9 system is a suitable gene targeting editing tool to filamentous fungus Monascus pilosus.Applied microbiology and biotechnology · 2024Article
- Oxidative stress drives mutagenesis through transcription-coupled repair in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Non-B DNA-Forming Motifs Promote Mfd-Dependent Stationary-Phase Mutagenesis inMicroorganisms · 2021Article
- Bacterial phenotypic heterogeneity in DNA repair and mutagenesis.Biochemical Society transactions · 2020Review
- Dual functionality of the amyloid protein TasA in Bacillus physiology and fitness on the phylloplane.Nature communications · 2020Article
- Role of Mfd and GreA in Bacillus subtilis Base Excision Repair-Dependent Stationary-Phase Mutagenesis.Journal of bacteriology · 2020Article
- Quorum sensing modulates the formation of virulent Legionella persisters within infected cells.Nature communications · 2019Article
- Roles ofJournal of bacteriology · 2019Article
- Mfd protects against oxidative stress in Bacillus subtilis independently of its canonical function in DNA repair.BMC microbiology · 2019Article
- Transcriptome and Volatilome Analysis During Growth ofFrontiers in microbiology · 2019Article
- Cyclic di-GMP Positively Regulates DNA Repair in Vibrio cholerae.Journal of bacteriology · 2018Article
- LC-MS/MS proteomic analysis of starved Bacillus subtilis cells overexpressing ribonucleotide reductase (nrdEF): implications in stress-associated mutagenesis.Current genetics · 2018Article
- Role of Ribonucleotide Reductase in Bacillus subtilis Stress-Associated Mutagenesis.Journal of bacteriology · 2017Article
- Implementation of a loss-of-function system to determine growth and stress-associated mutagenesis in Bacillus subtilis.PloS one · 2017Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
unlabelledReactive oxygen species (ROS) promote the synthesis of the DNA lesion 8-oxo-G, whose mutagenic effects are counteracted in distinct organisms by the DNA glycosylase MutM. We report here that in Bacillus subtilis, mutM is expressed during the exponential and stationary phases of growth. In agreement with this expression pattern, results of a Western blot analysis confirmed the presence of MutM in both stages of growth. In comparison with cells of a wild-type strain, cells of B. subtilis lacking MutM increased their spontaneous mutation frequency to Rif(r) and were more sensitive to the ROS promoter agents hydrogen peroxide and 1,1'-dimethyl-4,4'-bipyridinium dichloride (Paraquat). However, despite MutM's proven participation in preventing ROS-induced-DNA damage, the expression of mutM was not induced by hydrogen peroxide, mitomycin C, or NaCl, suggesting that transcription of this gene is not under the control of the RecA, PerR, or σ(B) regulons. Finally, the role of MutM in stationary-phase-associated mutagenesis (SPM) was investigated in the strain B. subtilis YB955 (hisC952 metB5 leuC427). Results revealed that under limiting growth conditions, a mutM knockout strain significantly increased the amount of stationary-phase-associated his, met, and leu revertants produced. In summary, our results support the notion that the absence of MutM promotes mutagenesis that allows nutritionally stressed B. subtilis cells to escape from growth-limiting conditions. IMPORTANCE: The present study describes the role played by a DNA repair protein (MutM) in protecting the soil bacterium Bacillus subtilis from the genotoxic effects induced by reactive oxygen species (ROS) promoter agents. Moreover, it reveals that the genetic inactivation of mutM allows nutritionally stressed bacteria to escape from growth-limiting conditions, putatively by a mechanism that involves the accumulation and error-prone processing of oxidized DNA bases.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.