ArticleMolecular microbiology2015
The intracellular parasite Toxoplasma gondii depends on the synthesis of long-chain and very long-chain unsaturated fatty acids not supplied by the host cell.
Article in Molecular microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 71 citations in OpenAlex.
- Transporters, an important but poorly studied area of Toxoplasma gondii.Parasites & vectors · 2026Review
- A genome-wide CRISPR screen identifies GRA38 as a key regulator of lipid homeostasis during Toxoplasma gondii adaptation to lipid-rich conditions.Nature communications · 2025Article
- Global profiling of polyketide synthases in facultative multicellular eukaryotes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A P5-ATPase, TgFLP12, diverging from plant chloroplast lipid transporters mediates apicoplast fatty export in Toxoplasma.Nature communications · 2025Article
- A genome-wide CRISPR screen identifies GRA38 as a key regulator of lipid homeostasis duringResearch square · 2025Article
- N-(9-Acridinyl) Amino Acid Derivatives: Synthesis and In Vitro Evaluation of Anti-Pharmaceutics · 2025Article
- The apicoplast localized isocitrate dehydrogenase is needed forFrontiers in cellular and infection microbiology · 2025Article
- Mitochondria of a human skeletal muscle cell line associate with the parasitophorous vacuoles of three archetypal lineages ofFEMS microbes · 2025Article
- Glabridin exhibits potent inhibitory effects against Toxoplasma gondii in vitro and in vivo.Parasites & vectors · 2024Article
- An endoplasmic reticulum localized acetyl-CoA transporter is required for efficient fatty acid synthesis inOpen biology · 2024Article
- Biological characteristics of a new long-chain fatty acid transport protein 1 from Trichinella spiralis and its participation in lipid metabolism, larval moulting, and development.Veterinary research · 2024Article
- A fatty acid elongase complex regulates cell membrane integrity and septin-dependent host infection by the rice blast fungus.Molecular plant pathology · 2024Article
- The MoLfa1 Protein Regulates Fungal Development and Septin Ring Formation inInternational journal of molecular sciences · 2024Article
- Lipid metabolism: the potential targets for toxoplasmosis treatment.Parasites & vectors · 2024Review
- Omega-3 and omega-6 polyunsaturated fatty acids and their potential therapeutic role in protozoan infections.Frontiers in immunology · 2024Review
- Dissection of Besnoitia besnoiti intermediate host life cycle stages: From morphology to gene expression.PLoS pathogens · 2022Article
- Glycosylation Analysis of Feline Small Intestine FollowingAnimals : an open access journal from MDPI · 2022Article
- Toxoplasma metabolic flexibility in different growth conditions.Trends in parasitology · 2022Review
- Experimental infection of Mongolian gerbils withVeterinary research forum : an international quarterly journal · 2022Article
- Ceramide biosynthesis is critical for establishment of the intracellular niche of Toxoplasma gondii.Cell reports · 2022Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
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Abstract
Apicomplexa are parasitic protozoa that cause important human diseases including malaria, cryptosporidiosis and toxoplasmosis. The replication of these parasites within their target host cell is dependent on both salvage as well as de novo synthesis of fatty acids. In Toxoplasma gondii, fatty acid synthesis via the apicoplast-localized FASII is essential for pathogenesis, while the role of two other fatty acid biosynthetic complexes remains unclear. Here, we demonstrate that the ER-localized fatty acid elongation (ELO) complexes are essential for parasite growth. Conditional knockdown of the nonredundant hydroxyacyl-CoA dehydratase and enoyl-CoA reductase enzymes in the ELO pathway severely repressed intracellular parasite growth. (13) C-glucose and (13) C-acetate labeling and comprehensive lipidomic analyses of these mutants showed a selective defect in synthesis of unsaturated long and very long-chain fatty acids (LCFAs and VLCFAs) and depletion of phosphatidylinositol and phosphatidylethanolamine species containing unsaturated LCFAs and VLCFAs. This requirement for ELO pathway was bypassed by supplementing the media with specific fatty acids, indicating active but inefficient import of host fatty acids. Our experiments highlight a gap between the fatty acid needs of the parasite and availability of specific fatty acids in the host cell that the parasite has to close using a dedicated synthesis and modification pathway.
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