Evidence map›Paper›PMID 2582474›Full record

ArticleCell and tissue research1989

Stage-specific localization of cytoskeletal actin mRNA in murine seminiferous tubules and intestinal epithelia as demonstrated by in-situ hybridization.

S Sakiyama, Y Nakamura, K Tokunaga, H Takazawa, Y Ohwaki, T Nagano

Abstract read
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In one paragraph

Article in Cell and tissue research, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 11 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

S SakiyamaDivision of Biochemistry, Chiba Cancer Center Research Institute, Japan.
Y Nakamura
K Tokunaga
H Takazawa
Y Ohwaki
T Nagano
Chiba Cancer Center · JPChiba Hospital · JPChiba University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In-situ hybridization experiments have been performed using isoactin (beta and gamma)-specific riboprobes in various tissues of the rat and mouse. Distribution of the grains of actin mRNAs for both beta and gamma types was similar throughout sections of the rat testis. Although both mRNAs were evenly distributed in the seminiferous tubule, extremely heavy labeling was observed in about 10% of the seminiferous tubules that could be identified as stage XII of spermatogenesis. At high magnification, grains of the mRNA were found in the cytoplasm of elongating spermatids and in the Sertoli cell cytoplasm at the adluminal side. Much higher density of the grains of mRNA was observed in the neck region of the spermatids at stage XII. Thus, the dense distribution of cytoskeletal actin mRNAs is stage-specific in the tubule during spermatogenesis in the rat. The high expression of both beta and gamma actin mRNAs was also observed in the epithelial cells of the intestinal crypts.

Indexed as

SpermatogenesisActinsAnimalsIntestinal MucosaIntestinesMaleMiceMice, Inbred C57BLNucleic Acid HybridizationRatsRats, Inbred StrainsRNA, MessengerSeminiferous TubulesTestisActinsRNA, Messenger

Identifiers

PMID2582474
OpenAlexW2006986031

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.