ArticleEuropean journal of immunology2015
IL-3 synergises with basophil-derived IL-4 and IL-13 to promote the alternative activation of human monocytes.
Article in European journal of immunology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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Who cites it
27 citing papers in PubMed, 44 citations in OpenAlex.
- Elucidating Immune Cell Mediated Causal Pathways Linking Blood Metabolites to Major Depressive Disorder: A Mediation Mendelian Randomization Analysis.Brain and behavior · 2026Article
- Basophils induce protumorigenic cytokines from A549 lung adenocarcinoma via mechanisms requiring IgE, galectin-3, and IL-3 priming.Journal of leukocyte biology · 2025Article
- A Maximum-Use Trial of Ruxolitinib Cream in Children Aged 2-11 Years with Moderate to Severe Atopic Dermatitis.American journal of clinical dermatology · 2025Article
- IL-3: key orchestrator of inflammation.Frontiers in immunology · 2024Review
- Interleukin-13 (IL-13)-A Pleiotropic Cytokine Involved in Wound Healing and Fibrosis.International journal of molecular sciences · 2023Review
- Establishing a novel and sensitive assay for bioactivity determination of anti-CD25 antibodies.Heliyon · 2023Article
- M-MDSCFrontiers in immunology · 2023Article
- Basophils beyond allergic and parasitic diseases.Frontiers in immunology · 2023Review
- IL-3-Induced Immediate Expression of c-Cells · 2022Article
- The S1 Subunit of the SARS-CoV-2 Spike Protein Activates Human Monocytes to Produce Cytokines Linked to COVID-19: Relevance to Galectin-3.Frontiers in immunology · 2022Article
- Evaluation of two different vaccine platforms for immunization against melioidosis and glanders.Frontiers in microbiology · 2022Article
- Basophils from allergy to cancer.Frontiers in immunology · 2022Review
- Basophils control T cell priming through soluble mediators rather than antigen presentation.Frontiers in immunology · 2022Review
- Basophils as a potential therapeutic target in cancer.Journal of Zhejiang University. Science. B · 2021Review
- CSF1R defines the mononuclear phagocyte system lineage in human blood in health and COVID-19.Immunotherapy advances · 2021Article
- Sputum Gene Expression Reveals Dysregulation of Mast Cells and Basophils in Eosinophilic COPD.International journal of chronic obstructive pulmonary disease · 2021Article
- Is There a Role for Basophils in Cancer?Frontiers in immunology · 2020Review
- Innate Immune Modulation by GM-CSF and IL-3 in Health and Disease.International journal of molecular sciences · 2019Review
- Immunosuppressive therapy with rituximab in common variable immunodeficiency.Clinical and molecular allergy : CMA · 2019Review
- The Intriguing Role of Interleukin 13 in the Pathophysiology of Asthma.Frontiers in pharmacology · 2019Review
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
Basophil-derived IL-4 is involved in the alternative activation of mouse monocytes, as recently shown in vivo. Whether this applies to human basophils and monocytes has not been established yet. Here, we sought to characterise the interaction between basophils and monocytes and identify the molecular determinants. A basophil-monocyte co-culture model revealed that IL-3 and basophil-derived IL-4 and IL-13 induced monocyte production of CCL17, a marker of alternative activation. Critically, IL-3 and IL-4 acted directly on monocytes to induce CCL17 production through histone H3 acetylation, but did not increase the recruitment of STAT5 or STAT6. Although freshly isolated monocytes did not express the IL-3 receptor α chain (CD123), and did not respond to IL-3 (as assessed by STAT5 phosphorylation), the overnight incubation with IL-4 (especially if associated with IL-3) upregulated CD123 expression. IL-3-activated JAK2-STAT5 pathway inhibitors reduced the CCL17 production in response to IL-3 and IL-4, but not to IL-4 alone. Interestingly, monocytes isolated from allergen-sensitised asthmatic patients exhibited a higher expression of CD123. Taken together, our data show that the JAK2-STAT5 pathway modulates both basophil and monocyte effector responses. The coordinated activation of STAT5 and STAT6 may have a major impact on monocyte alternative activation in vitro and in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.