ArticleeLife2015
A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila.
Article in eLife, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 278 papers.
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Who cites it
278 citing papers in PubMed.
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- Hippo signaling regulates cuticle pigmentation and dopamine metabolism in Drosophila.PLoS genetics · 2026Article
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- Phage display-mediated immuno-PCR to detect low-abundance secreted proteins inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Hbs and Rst adhesion molecules provide a regional code that regulates cell elimination during epithelial remodeling.iScience · 2026Article
- Selective regulation of transsynaptic alignment and postsynaptic assembly by a novel NCAM family synaptic adhesion molecule.bioRxiv : the preprint server for biology · 2026Article
- Pro-tumoral CaScientific reports · 2026Article
- Mmp2 regulates basement membrane remodeling and dedifferentiation of the visceral musculature during Drosophila metamorphosis.Scientific reports · 2026Article
- To Be or Not to Be an Oocyte: Msps/XMAP215 Controls Oocyte Cell Fate in thebioRxiv : the preprint server for biology · 2026Article
- Tissue-specific role of dTrmO in threonine decoding during Drosophila melanogaster development.Biological research · 2026Article
- Beat II and Side IV keep migrating longitudinal visceral muscle precursor cells on their substratum in Drosophila.Journal of cell science · 2026Article
- Inferring chromatin architecture at a single locus through probabilistic in situ DNA localization.Nature communications · 2026Article
- Analysis of Muscle Structure Using Fluorescently Tagged Proteins in Drosophila melanogaster Femur Muscles.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Genetic interaction ofFrontiers in neuroscience · 2026Article
- Jub-induced phosphorylation of Warts inhibits its activity and recruitment into biomolecular condensates.Cell reports · 2025Article
218 more citing papers are in PubMed but not listed here.
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Authors and funding
17 authors.
Funding
Abstract
Here, we document a collection of ∼7434 MiMIC (Minos Mediated Integration Cassette) insertions of which 2854 are inserted in coding introns. They allowed us to create a library of 400 GFP-tagged genes. We show that 72% of internally tagged proteins are functional, and that more than 90% can be imaged in unfixed tissues. Moreover, the tagged mRNAs can be knocked down by RNAi against GFP (iGFPi), and the tagged proteins can be efficiently knocked down by deGradFP technology. The phenotypes associated with RNA and protein knockdown typically correspond to severe loss of function or null mutant phenotypes. Finally, we demonstrate reversible, spatial, and temporal knockdown of tagged proteins in larvae and adult flies. This new strategy and collection of strains allows unprecedented in vivo manipulations in flies for many genes. These strategies will likely extend to vertebrates.
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