ArticlePloS one2015
An in vivo metabolic approach for deciphering the product specificity of glycerate kinase proves that both E. coli's glycerate kinases generate 2-phosphoglycerate.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- Microbial Ethylene Glycol Metabolism: Pathways, Metabolic Engineering, and PET Upcycling.Current microbiology · 2026Review
- From screening to sequencing: Investigating the monoethylene glycol (MEG) biodegradation byBiotechnology reports (Amsterdam, Netherlands) · 2026Article
- From plastic waste to bioprocesses: Using ethylene glycol from polyethylene terephthalate biodegradation to fuelMetabolic engineering communications · 2024Article
- Global regulation via modulation of ribosome pausing by the ABC-F protein EttA.Nature communications · 2024Article
- Novel aspects of ethylene glycol catabolism.Applied microbiology and biotechnology · 2024Review
- Multiple Roles of Glycerate Kinase-From Photorespiration to Gluconeogenesis, CInternational journal of molecular sciences · 2024Review
- Evaluation of Cellular Responses of HeterotrophicMicroorganisms · 2024Article
- Directed evolution of alditol oxidase for the production of optically pure D-glycerate from glycerol in the engineered Escherichia coli.Journal of industrial microbiology & biotechnology · 2021Article
- Construction of an alternative glycerol-utilization pathway for improved β-carotene production in Escherichia coli.Journal of industrial microbiology & biotechnology · 2018Article
- Engineering Escherichia coli to grow constitutively on D-xylose using the carbon-efficient Weimberg pathway.Microbiology (Reading, England) · 2018Article
- Article
- Temperature-Photoperiod Interaction in Rice Phenology for Climate Adaptation: Insights Into Glycerate-Associated Metabolic Responses.Physiologia plantarumArticle
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Authors and funding
8 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Apart from addressing humanity's growing demand for fuels, pharmaceuticals, plastics and other value added chemicals, metabolic engineering of microbes can serve as a powerful tool to address questions concerning the characteristics of cellular metabolism. Along these lines, we developed an in vivo metabolic strategy that conclusively identifies the product specificity of glycerate kinase. By deleting E. coli's phosphoglycerate mutases, we divide its central metabolism into an 'upper' and 'lower' metabolism, each requiring its own carbon source for the bacterium to grow. Glycerate can serve to replace the upper or lower carbon source depending on the product of glycerate kinase. Using this strategy we show that while glycerate kinase from Arabidopsis thaliana produces 3-phosphoglycerate, both E. coli's enzymes generate 2-phosphoglycerate. This strategy represents a general approach to decipher enzyme specificity under physiological conditions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.