Evidence map›Paper›PMID 25822693›Full record

ArticleNature materials2015

Biodegradable silicon nanoneedles delivering nucleic acids intracellularly induce localized in vivo neovascularization.

C Chiappini, E De Rosa, J O Martinez, X Liu, J Steele, M M Stevens, E Tasciotti

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature materials, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 136 papers.

0numbers the graph read from it
0cells of the map it votes in
136citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

136 citing papers in PubMed.

  1. Overview of Delivery Methods for Gene Editing.Methods in molecular biology (Clifton, N.J.) · 2027
    Review
  2. Review
  3. Poking Pluripotency: Nanoinjection Into Human iPSCs.Advanced materials (Deerfield Beach, Fla.) · 2026
    Article
  4. Article
  5. Just a little prick: careful cell contacts enabled by ceramic nanostraws.Pflugers Archiv : European journal of physiology · 2026
    Article
  6. Smart micro/nanoneedles for gene delivery.Iranian journal of basic medical sciences · 2026
    Review
  7. Article
  8. Article
  9. Article
  10. Review
  11. Review
  12. Article
  13. Article
  14. Article
  15. Review
  16. Review
  17. Article
  18. Review
  19. Review
  20. Article

76 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

C Chiappini1] Department of Materials, Imperial College London, London, SW6 7PB, UK [2] Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London, SW6 7PB, UK.
E De RosaDepartment of Nanomedicine, Houston Methodist Research Institute, Houston, Texas 77030, USA.
J O MartinezDepartment of Nanomedicine, Houston Methodist Research Institute, Houston, Texas 77030, USA.
X LiuDepartment of Nanomedicine, Houston Methodist Research Institute, Houston, Texas 77030, USA.
J Steele1] Department of Materials, Imperial College London, London, SW6 7PB, UK [2] Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London, SW6 7PB, UK.
M M Stevens1] Department of Materials, Imperial College London, London, SW6 7PB, UK [2] Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, London, SW6 7PB, UK.
E TasciottiDepartment of Nanomedicine, Houston Methodist Research Institute, Houston, Texas 77030, USA.

Funding

TRANS-NETWORK PROJECTSU54CA143837 · NCI · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI FERRARI, MAURO · 2009 to 2014
$10.8M
Leukocyte Membrane Cloaked Carriers to Target and Overcome Tumor VasculatureR21CA173579 · NCI · METHODIST HOSPITAL RESEARCH INSTITUTE · PI TASCIOTTI, ENNIO · 2013 to 2014
$371k
Stem Cell-Directed Nanotheranostics for Cancer InterventionF31CA154119 · NCI · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI MARTINEZ, JONATHAN OTTO · 2011 to 2014
$64k
European Research Council 616417NCI NIH HHS 1R21CA173579-01A1NCI NIH HHS 5F31CA154119-02NCI NIH HHS 5U54CA143837NCI NIH HHS F31 CA154119NCI NIH HHS R21 CA173579NCI NIH HHS U54 CA143837Wellcome Trust 098411
6 · The paper itself

Abstract

The controlled delivery of nucleic acids to selected tissues remains an inefficient process mired by low transfection efficacy, poor scalability because of varying efficiency with cell type and location, and questionable safety as a result of toxicity issues arising from the typical materials and procedures employed. High efficiency and minimal toxicity in vitro has been shown for intracellular delivery of nuclei acids by using nanoneedles, yet extending these characteristics to in vivo delivery has been difficult, as current interfacing strategies rely on complex equipment or active cell internalization through prolonged interfacing. Here, we show that a tunable array of biodegradable nanoneedles fabricated by metal-assisted chemical etching of silicon can access the cytosol to co-deliver DNA and siRNA with an efficiency greater than 90%, and that in vivo the nanoneedles transfect the VEGF-165 gene, inducing sustained neovascularization and a localized sixfold increase in blood perfusion in a target region of the muscle.

Indexed as

NanostructuresNeedlesNeovascularization, PhysiologicPlasmidsSiliconTransfectionVascular Endothelial Growth Factor AAnimalsHumansMiceMuscle, SkeletalSiliconVascular Endothelial Growth Factor AVEGFA protein, human

Identifiers

PMID25822693
PMCPMC4538992

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.