ArticlePloS one2015
Germline-transmitted genome editing in Arabidopsis thaliana Using TAL-effector-nucleases.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- The hypoxic niche enclosing the shoot apical meristem is shaped by a combination of morphological features and metabolic activity.Molecular plant · 2026Article
- The trehalose 6-phosphate pathway coordinates dynamic changes at the shoot apical meristem in Arabidopsis thaliana.Plant physiology · 2025Article
- CLAVATA3 Signaling Buffers Arabidopsis Shoot Apical Meristem Activity in Response to Photoperiod.International journal of molecular sciences · 2024Article
- Functional Allele Validation by Gene Editing to Leverage the Wealth of Genetic Resources for Crop Improvement.International journal of molecular sciences · 2022Review
- Aiming for the top: non-cell autonomous control of shoot stem cells in Arabidopsis.Journal of plant research · 2020Review
- Strategies to Increase On-Target and Reduce Off-Target Effects of the CRISPR/Cas9 System in Plants.International journal of molecular sciences · 2019Review
- Plant Genome Engineering for Targeted Improvement of Crop Traits.Frontiers in plant science · 2019Review
- Efficient Multiplex Genome Editing Induces Precise, and Self-Ligated Type Mutations in Tomato Plants.Frontiers in plant science · 2018Article
- A Multipurpose Toolkit to Enable Advanced Genome Engineering in Plants.The Plant cell · 2017Article
- Heritability of targeted gene modifications induced by plant-optimized CRISPR systems.Cellular and molecular life sciences : CMLS · 2017Review
- CRISPR/Cas9-mediated targeted mutagenesis in grape.PloS one · 2017Article
- Plant genome editing with TALEN and CRISPR.Cell & bioscience · 2017Review
- TALEN-mediated genome-editing approaches in the liverwortPlant methods · 2017Article
- A Defect in DNA Ligase4 Enhances the Frequency of TALEN-Mediated Targeted Mutagenesis in Rice.Plant physiology · 2016Article
- Development of germ-line-specific CRISPR-Cas9 systems to improve the production of heritable gene modifications in Arabidopsis.Plant biotechnology journal · 2016Article
- Genome-Editing Technologies for Enhancing Plant Disease Resistance.Frontiers in plant science · 2016Review
- Correction: Germline-Transmitted Genome Editing in Arabidopsis thaliana Using TAL-Effector-Nucleases.PloS one · 2015Article
Corrections and comments
- Erratum issuedCorrection: Germline-Transmitted Genome Editing in Arabidopsis thaliana Using TAL-Effector-Nucleases.2015 · Manavella, Pablo [corrected to Manavella, Pablo A]
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription activator-like effector nucleases (TALENs) are custom-made bi-partite endonucleases that have recently been developed and applied for genome engineering in a wide variety of organisms. However, they have been only scarcely used in plants, especially for germline-modification. Here we report the efficient creation of small, germline-transmitted deletions in Arabidopsis thaliana via TALENs that were delivered by stably integrated transgenes. Using meristem specific promoters to drive expression of two TALEN arms directed at the CLV3 coding sequence, we observed very high phenotype frequencies in the T2 generation. In some instances, full CLV3 loss-of-function was already observed in the T1 generation, suggesting that transgenic delivery of TALENs can cause highly efficient genome modification. In contrast, constitutive TALEN expression in the shoot apical meristem (SAM) did not cause additional phenotypes and genome re-sequencing confirmed little off-target effects, demonstrating exquisite target specificity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.