ArticlePloS one2015
Genomic DNA hypomethylation is associated with neural tube defects induced by methotrexate inhibition of folate metabolism.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Excessive DNA Double-Strand Breaks-Associated 3D Genome Reorganization Contributes to Neural Tube Defects with Folate Deficiency.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Micronutrient regulation of the DNA methylome.Frontiers in epigenetics and epigenomics · 2024Review
- DNA methylation profile of genes involved in inflammation and autoimmunity correlates with vascular function in morbidly obese adults.Epigenetics · 2022Article
- Abnormal methylation caused by folic acid deficiency in neural tube defects.Open life sciences · 2022Review
- Article
- Adipose Tissue Hypoxia Correlates with Adipokine Hypomethylation and Vascular Dysfunction.Biomedicines · 2021Article
- Methylene tetrahydrofolate reductase A1298C polymorphisms influence the adult sequelae of chemotherapy in childhood-leukemia survivors.PloS one · 2021Article
- Review
- Deoxyuracil in DNA in health and disease.Current opinion in clinical nutrition and metabolic care · 2020Review
- Transcriptome changes underlie alterations in behavioral traits in different types of chicken.Journal of animal science · 2020Article
- Casp8 hypomethylation and neural tube defects in association with polycyclic aromatic hydrocarbon exposure.Clinical epigenetics · 2019Article
- Deoxyuracil in DNA and disease: Genomic signal or managed situation?DNA repair · 2019Review
- Interaction between endothelial nitric oxide synthase rs1799983, cholesteryl ester-transfer protein rs708272 and angiopoietin-like protein 8 rs2278426 gene variants highly elevates the risk of type 2 diabetes mellitus and cardiovascular disease.Cardiovascular diabetology · 2018Article
- Folic Acid Exposure Rescues Spina Bifida Aperta Phenotypes in Human Induced Pluripotent Stem Cell Model.Scientific reports · 2018Article
- Global methylation in relation to methotrexate-induced oral mucositis in children with acute lymphoblastic leukemia.PloS one · 2018Article
- S-Adenosyl Methionine and Transmethylation Pathways in Neuropsychiatric Diseases Throughout Life.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2018Review
- Effect of methotrexate/vitamin BEpigenomics · 2017Article
- Methotrexate and Valproic Acid Affect Early Neurogenesis of Human Amniotic Fluid Stem Cells from Myelomeningocele.Stem cells international · 2017Article
- A mutation in the tuft mouse disrupts TET1 activity and alters the expression of genes that are crucial for neural tube closure.Disease models & mechanisms · 2016Article
- Glucose Tolerance,Journal of diabetes research · 2016Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA methylation is thought to be involved in the etiology of neural tube defects (NTDs). However, the exact mechanism between DNA methylation and NTDs remains unclear. Herein, we investigated the change of methylation in mouse model of NTDs associated with folate dysmetabolism by use of ultraperformance liquid chromatography tandem mass spectrometry (UPLC/MS/MS), liquid chromatography-electrospray ionization tandem mass spectrometry (LC-MS/MS), microarray, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and Real time quantitative PCR. Results showed that NTD neural tube tissues had lower concentrations of 5-methyltetrahydrofolate (5-MeTHF, P = 0.005), 5-formyltetrahydrofolate (5-FoTHF, P = 0.040), S-adenosylmethionine (SAM, P = 0.004) and higher concentrations of folic acid (P = 0.041), homocysteine (Hcy, P = 0.006) and S-adenosylhomocysteine (SAH, P = 0.045) compared to control. Methylation levels of genomic DNA decreased significantly in the embryonic neural tube tissue of NTD samples. 132 differentially methylated regions (35 low methylated regions and 97 high methylated regions) were selected by microarray. Two genes (Siah1b, Prkx) in Wnt signal pathway demonstrated lower methylated regions (peak) and higher expression in NTDs (P<0.05; P<0.05). Results suggest that DNA hypomethylation was one of the possible epigenetic variations correlated with the occurrence of NTDs induced by folate dysmetabolism and that Siah1b, Prkx in Wnt pathway may be candidate genes for NTDs.
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