ArticlePloS one2015
Structural and immunological effects of skin cryoablation in a mouse model.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Successful Treatment of Localized Eruptive Pruritic Papular Porokeratosis with A Single Session of Cryotherapy: A Case Report.Clinical, cosmetic and investigational dermatology · 2026Article
- Tissue Ablation: Applications and Perspectives.Advanced materials (Deerfield Beach, Fla.) · 2024Review
- An Immune-Based Therapeutical Approach in an Elderly Patient With Fixed Cutaneous Sporotrichosis.Cureus · 2024Article
- Current applications of tumor local ablation (TLA) combined with immune checkpoint inhibitors in breast cancer treatment.Cancer drug resistance (Alhambra, Calif.) · 2024Review
- The State of the Art in Transcriptomics and Proteomics of Clinically RelevantJournal of fungi (Basel, Switzerland) · 2023Review
- Cryoablation and Immunotherapy: An Enthralling Synergy for Cancer Treatment.Current oncology (Toronto, Ont.) · 2023Article
- In-situ cryo-immune engineering of tumor microenvironment with cold-responsive nanotechnology for cancer immunotherapy.Nature communications · 2023Article
- Sporotrichosis: A Review of a Neglected Disease in the Last 50 Years in Brazil.Microorganisms · 2022Review
- Changes in and effects of Kupffer cells on residual tumor after cryoablation in rabbit hepatic VX2 tumor.International journal of clinical and experimental pathology · 2021Article
- Liquid Nitrogen-Based Cryoablation in In Vivo Porcine Tissue: A Pilot Study.Asian Pacific journal of cancer prevention : APJCP · 2020Article
- Anti-inflammatory role of 15-lipoxygenase contributes to the maintenance of skin integrity in mice.Scientific reports · 2018Article
- Cryosurgery for the treatment of cutaneous sporotrichosis in four pregnant women.PLoS neglected tropical diseases · 2018Article
- [The aged scalp : A dermato-oncological focus point].Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete · 2017Review
- [Actinic keratoses].Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete · 2016Review
- Distant Resolution of Actinic Keratosis following Cryosurgery: An Unusual Phenomenon.Case reports in dermatologyArticle
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryoablation is therapeutically applied for various disorders in several organs, and skin diseases are typical targets as this cryotherapy has been widely used for viral warts, benign tumors, and actinic keratosis. The main mechanisms of cryoablation consist of direct freezing effect on skin constituents, thrombosis formation in microcirculation, and subsequent immunological responses. Among them, however, the immunological mechanism remains unelucidated, and it is an issue how the direct freezing injury induces immunological consequences. We established a mouse cryoablation model with liquid nitrogen applied to the shaved back skin, and used this system to study the immunological excitement. After application of liquid nitrogen, the thermal decrease ratio was -25°C/sec or less and the lowest temperature was less than -100°C, which was sufficient to induce ulceration. Destruction of cornified layer and necrosis of epidermal cells were observed in transmission electron microscopy image, and increased transepidermal water loss and skin permeability were detected by the functional measurements. By flow cytometry, antigen-presenting dendritic cells (DCs), including PDCA1+B220+CD19- plasmacytoid DCs (pDCs) and CD11c+ myeloid DCs, as well as neutrophils and macrophages were increased in subcutaneous tissue. In parallel, the mRNA expressions of interferon α1 which are known as pDC-producing cytokines, was elevated. We also found marked degranulation of mast cells, providing a possibility that released histamine attracts pDCs. Finally, FITC migration assay revealed that pDCs and CD11c+ DCs emigrated from the cryoablated skin to the draining lymph nodes. Our study suggests that cryoablation induces destruction of the barrier/epidermis, accumulation of pDCs and CD11c+ DCs to the skin, and migration of DCs to regional lymph nodes. Viral elements or tumor cell lysates released from damaged keratinocytes may stimulate the DCs, thereby leading to antiviral or antitumor effect.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.