ArticleBiochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology
Teaching bioprocess engineering to undergraduates: Multidisciplinary hands-on training in a one-week practical course.
Article in Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 15 citations in OpenAlex.
- RNA thermometers as temperature-dependent tools for enhanced recombinant protein production in precision fermentation.Microbial cell factories · 2026Article
- Making GFP count: a validated framework for absolute protein quantification in precision fermentation.Applied microbiology and biotechnology · 2026Article
- Reforming pedagogy in biotechnology pharmaceutics education: a narrative review of emerging teaching strategies.Frontiers in psychology · 2026Review
- Glucoselipid Biosurfactant Biosynthesis Operon ofMicroorganisms · 2025Article
- Genetic Code Expansion for Controlled Surfactin Production in a High Cell-DensityMicroorganisms · 2025Article
- The influence of growth rate-controlling feeding strategy on the surfactin production in Bacillus subtilis bioreactor processes.Microbial cell factories · 2024Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bioprocess engineering is a highly interdisciplinary field of study which is strongly benefited by practical courses where students can actively experience the interconnection between biology, engineering, and physical sciences. This work describes a lab course developed for 2nd year undergraduate students of bioprocess engineering and related disciplines, where students are challenged with a real-life bioprocess-engineering application, the production of recombinant protein in a fed-batch process. The lab course was designed to introduce students to the subject of operating and supervising an experiment in a bioreactor, along with the analysis of collected data and a final critical evaluation of the experiment. To provide visual feedback of the experimental outcome, the organism used during class was Escherichia coli which carried a plasmid to recombinantly produce enhanced green fluorescent protein (eGFP) upon induction. This can easily be visualized in both the bioreactor and samples by using ultraviolet light. The lab course is performed with bioreactors of the simplest design, and is therefore highly flexible, robust and easy to reproduce. As part of this work the implementation and framework, the results, the evaluation and assessment of student learning combined with opinion surveys are presented, which provides a basis for instructors intending to implement a similar lab course at their respective institution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.