ArticleNucleic acids research2015
RNA polymerase II pausing can be retained or acquired during activation of genes involved in the epithelial to mesenchymal transition.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Transcriptional responses of cancer cells to heat shock-inducing stimuli involve amplification of robust HSF1 binding.Nature communications · 2023Article
- RNA polymerase II pausing in development: orchestrating transcription.Open biology · 2022Review
- Hedgehog/GLI Signaling Pathway: Transduction, Regulation, and Implications for Disease.Cancers · 2021Review
- Targeting the Transcriptome Through Globally Acting Components.Frontiers in genetics · 2021Review
- PTEN modulates gene transcription by redistributing genome-wide RNA polymerase II occupancy.Human molecular genetics · 2019Article
- The Histone Deacetylase SIRT6 Restrains Transcription Elongation via Promoter-Proximal Pausing.Molecular cell · 2019Article
- Article
- Characterizing functional consequences of DNA copy number alterations in breast and ovarian tumors by spaceMap.Journal of genetics and genomics = Yi chuan xue bao · 2018Article
- The calcium channel proteins ORAI3 and STIM1 mediate TGF-β inducedOncotarget · 2018Article
- Pharmacological perturbation of CDK9 using selective CDK9 inhibition or degradation.Nature chemical biology · 2018Article
- Direct HPV E6/Myc interactions induce histone modifications, Pol II phosphorylation, and hTERT promoter activation.Oncotarget · 2017Article
- A novel lnc-PCF promotes the proliferation of TGF-β1-activated epithelial cells by targeting miR-344a-5p to regulate map3k11 in pulmonary fibrosis.Cell death & disease · 2017Article
- Additional sex combs interacts with enhancer of zeste and trithorax and modulates levels of trimethylation on histone H3K4 and H3K27 during transcription of hsp70.Epigenetics & chromatin · 2017Article
- Multiplexed gene control reveals rapid mRNA turnover.Science advances · 2017Article
- The C/ebp-Atf response element (CARE) location reveals two distinct Atf4-dependent, elongation-mediated mechanisms for transcriptional induction of aminoacyl-tRNA synthetase genes in response to amino acid limitation.Nucleic acids research · 2016Article
- RNA polymerase II pausing as a context-dependent reader of the genome.Biochemistry and cell biology = Biochimie et biologie cellulaire · 2016Review
- Analysis of paired end Pol II ChIP-seq and short capped RNA-seq in MCF-7 cells.Genomics data · 2015Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Promoter-proximal RNA polymerase II (Pol II) pausing is implicated in the regulation of gene transcription. However, the mechanisms of pausing including its dynamics during transcriptional responses remain to be fully understood. We performed global analysis of short capped RNAs and Pol II Chromatin Immunoprecipitation sequencing in MCF-7 breast cancer cells to map Pol II pausing across the genome, and used permanganate footprinting to specifically follow pausing during transcriptional activation of several genes involved in the epithelial to mesenchymal transition (EMT). We find that the gene for EMT master regulator Snail (SNAI1), but not Slug (SNAI2), shows evidence of Pol II pausing before activation. Transcriptional activation of the paused SNAI1 gene is accompanied by a further increase in Pol II pausing signal, whereas activation of non-paused SNAI2 gene results in the acquisition of a typical pausing signature. The increase in pausing signal reflects increased transcription initiation without changes in Pol II pausing. Activation of the heat shock HSP70 gene involves pausing release that speeds up Pol II turnover, but does not change pausing location. We suggest that Pol II pausing is retained during transcriptional activation and can further undergo regulated release in a signal-specific manner.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.