ArticleApplied and environmental microbiology2015
Evaluation of Molecular Methods To Improve the Detection of Burkholderia pseudomallei in Soil and Water Samples from Laos.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- CRISPR-based environmental detection of Burkholderia pseudomallei identifies sanitation gaps and melioidosis risk in northeast Thailand.Nature communications · 2026Article
- Evaluation of culture- and PCR-based methods for detecting Burkholderia pseudomallei in soil samples in Thailand.PLoS neglected tropical diseases · 2026Article
- Identification ofMicrobiology spectrum · 2024Article
- Rational design of an acidic erythritol (ACER) medium for the enhanced isolation of the environmental pathogenFrontiers in microbiology · 2023Article
- Environmental Factors Associated With Soil Prevalence of the Melioidosis PathogenFrontiers in microbiology · 2022Article
- Enhanced melioidosis surveillance in patients attending four tertiary hospitals in Yangon, Myanmar.Epidemiology and infection · 2021Article
- Geographical distribution of Burkholderia pseudomallei in soil in Myanmar.PLoS neglected tropical diseases · 2021Article
- Using Land Runoff to Survey the Distribution and Genetic Diversity ofApplied and environmental microbiology · 2021Article
- Human Melioidosis.Clinical microbiology reviews · 2020Review
- Erythritol as a single carbon source improves cultural isolation of Burkholderia pseudomallei from rice paddy soils.PLoS neglected tropical diseases · 2019Article
- A cluster of melioidosis infections in hatchling saltwater crocodiles (Microbial genomics · 2019Article
- A Study ofThe American journal of tropical medicine and hygiene · 2019Article
- Rivers as carriers and potential sentinels for Burkholderia pseudomallei in Laos.Scientific reports · 2018Article
- Melioidosis in the Lao People's Democratic Republic.Tropical medicine and infectious disease · 2018Review
- Evaluation of consensus method for the culture ofWellcome open research · 2018Article
- Presence of B. thailandensis and B. thailandensis expressing B. pseudomallei-like capsular polysaccharide in Thailand, and their associations with serological response to B. pseudomallei.PLoS neglected tropical diseases · 2018Article
- Burkholderia pseudomallei in a lowland rice paddy: seasonal changes and influence of soil depth and physico-chemical properties.Scientific reports · 2017Article
- Hydrological connectivity and Burkholderia pseudomallei prevalence in wetland environments: investigating rice-farming community's risk of exposure to melioidosis in North-East Thailand.Environmental monitoring and assessment · 2017Article
- Multitarget Quantitative PCR Improves Detection and Predicts Cultivability of the Pathogen Burkholderia pseudomallei.Applied and environmental microbiology · 2017Article
- Utility of a Lateral Flow Immunoassay (LFI) to Detect Burkholderia pseudomallei in Soil Samples.PLoS neglected tropical diseases · 2016Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 4 countries.
Funding
Abstract
Burkholderia pseudomallei is the cause of melioidosis, a severe and potentially fatal disease of humans and animals. It is endemic in northern Australia and Southeast Asia and is found in soil and surface water. The environmental distribution of B. pseudomallei worldwide and within countries where it is endemic, such as the Lao People's Democratic Republic (Laos), remains unclear. However, this knowledge is important to our understanding of the ecology and epidemiology of B. pseudomallei and to facilitate public health interventions. Sensitive and specific methods to detect B. pseudomallei in environmental samples are therefore needed. The aim of this study was to compare molecular and culture-based methods for the detection of B. pseudomallei in soil and surface water in order to identify the optimal approach for future environmental studies in Laos. Molecular detection by quantitative real-time PCR (qPCR) was attempted after DNA extraction directly from soil or water samples or after an overnight enrichment step. The positivity rates obtained by qPCR were compared to those obtained by different culture techniques. The rate of detection from soil samples by qPCR following culture enrichment was significantly higher (84/100) than that by individual culture methods and all culture methods combined (44/100; P < 0.001). Similarly, qPCR following enrichment was the most sensitive method for filtered river water compared with the sensitivity of the individual methods and all individual methods combined. In conclusion, molecular detection following an enrichment step has proven to be a sensitive and reliable approach for B. pseudomallei detection in Lao environmental samples and is recommended as the preferred method for future surveys.
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