ArticleApplied and environmental microbiology2015
Lincomycin at Subinhibitory Concentrations Potentiates Secondary Metabolite Production by Streptomyces spp.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Discovery of natural products that modulate signaling in patient-derived cells.RSC chemical biology · 2026Article
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- Role of Metabolomics and Metagenomics in the Replacement of the High-Concentrate Diet with a High-Fiber Diet for Growing Yushan Pigs.Animals : an open access journal from MDPI · 2024Article
- High-intensity green light potentially activates the actinorhodin biosynthetic pathway in Streptomyces coelicolor A3(2).Archives of microbiology · 2023Article
- Phenomenological interpretations of the mechanism for the concentration-dependent positive effect of antibiotic lincomycin onApplied and environmental microbiology · 2023Article
- The Hormetic Effect Observed for Benzalkonium Chloride and Didecyldimethylammonium Chloride inMicroorganisms · 2023Article
- Small molecule inducers of actinobacteria natural product biosynthesis.Journal of industrial microbiology & biotechnology · 2023Review
- Elicitation for activation of the actinomycete genome's cryptic secondary metabolite gene clusters.RSC advances · 2023Review
- Enhancing chemical and biological diversity by co-cultivation.Frontiers in microbiology · 2023Review
- Review
- A Glossary for Chemical Approaches towards Unlocking the Trove of Metabolic Treasures inMolecules (Basel, Switzerland) · 2021Review
- Metabolomics and genomics in natural products research: complementary tools for targeting new chemical entities.Natural product reports · 2021Review
- Article
- Interaction between β-lactam antibiotic and phosphorus-accumulating organisms.Environmental science and pollution research international · 2021Article
- Piperacillin triggers virulence factor biosynthesis via the oxidative stress response inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- A Natural Product Chemist's Guide to Unlocking Silent Biosynthetic Gene Clusters.Annual review of biochemistry · 2021Review
- Characterization and complete genome analysis of Bacillus velezensis CB6 revealed ATP synthase subunit α against foodborne pathogens.Archives of microbiology · 2021Article
- Identification of a gene from Streptomyces rimosus M527 negatively affecting rimocidin biosynthesis and morphological differentiation.Applied microbiology and biotechnology · 2020Article
- Lincomycin-Induced Secondary Metabolism in Streptomyces lividans 66 with a Mutation in the Gene Encoding the RNA Polymerase Beta Subunit.Current microbiology · 2020Article
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5 authors.
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Abstract
Antibiotics have either bactericidal or bacteriostatic activity. However, they also induce considerable gene expression in bacteria when used at subinhibitory concentrations (below the MIC). We found that lincomycin, which inhibits protein synthesis by binding to the ribosomes of Gram-positive bacteria, was effective for inducing the expression of genes involved in secondary metabolism in Streptomyces strains when added to medium at subinhibitory concentrations. In Streptomyces coelicolor A3(2), lincomycin at 1/10 of its MIC markedly increased the expression of the pathway-specific regulatory gene actII-ORF4 in the blue-pigmented antibiotic actinorhodin (ACT) biosynthetic gene cluster, which resulted in ACT overproduction. Intriguingly, S. lividans 1326 grown in the presence of lincomycin at a subinhibitory concentration (1/12 or 1/3 of its MIC) produced abundant antibacterial compounds that were not detected in cells grown in lincomycin-free medium. Bioassay and mass spectrometry analysis revealed that some antibacterial compounds were novel congeners of calcium-dependent antibiotics. Our results indicate that lincomycin at subinhibitory concentrations potentiates the production of secondary metabolites in Streptomyces strains and suggest that activating these strains by utilizing the dose-response effects of lincomycin could be used to effectively induce the production of cryptic secondary metabolites. In addition to these findings, we also report that lincomycin used at concentrations for markedly increased ACT production resulted in alteration of the cytoplasmic protein (FoF1 ATP synthase α and β subunits, etc.) profile and increased intracellular ATP levels. A fundamental mechanism for these unique phenomena is also discussed.
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