ArticleThe Journal of surgical research2015
Epidermal growth factor mediated healing in stem cell-derived vocal fold mucosa.
Article in The Journal of surgical research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 13 citations in OpenAlex.
- Prospective Outcomes After Serial Platelet-Rich Plasma (PRP) Injection in Vocal Fold Scar and Sulcus.The Laryngoscope · 2024Trial
- Article
- Descriptive proteomics of paired human vocal fold and buccal mucosa tissue.Proteomics. Clinical applications · 2022Article
- Lack of Association between Epidermal Growth Factor or Its Receptor and Reflux Esophagitis, Barrett's Esophagus, and Esophageal Adenocarcinoma: A Case-Control Study.Disease markers · 2022Article
- Growth Factors, Reactive Oxygen Species, and Metformin-Promoters of the Wound Healing Process in Burns?International journal of molecular sciences · 2021Review
- Novel immortalized human vocal fold epithelial cell line: In vitro tool for mucosal biology.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Tissue engineering applications in otolaryngology-The state of translation.Laryngoscope investigative otolaryngology · 2020Review
- Prolactin may serve as a regulator to promote vocal fold wound healing.Bioscience reports · 2020Article
- An in vitro scaffold-free epithelial-fibroblast coculture model for the larynx.The Laryngoscope · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe goal of vocal fold wound healing is the reconstitution of functional tissue, including a structurally and functionally intact epithelium. Mechanisms underlying reepithelialization in vocal folds are not known, although it is suspected that healing involves the interplay between several growth factors. We used a three-dimensional human embryonic stem cell-derived model of vocal fold mucosa to examine the effects of one growth factor, exogenous epidermal growth factor (EGF), on wound healing. MATERIALS AND
methodsA scratch wound was created in the in vitro model. Rate of wound healing, epidermal growth factor receptor (EGFR) activation, and cell proliferation after injury were analyzed with and without application of both exogenous EGF and an EGFR inhibitor, gefitinib.
resultsWound repair after injury was significantly hastened by application of exogenous EGF (13.3 μm/h, ± 2.63) compared with absence of exogenous EGF (7.1 μm/h ± 2.84), but inhibited with concurrent addition of Gefitinib (5.2 μm/h, ± 2.23), indicating that EGF mediates wound healing in an EGFR-dependent manner. Immunohistochemistry revealed that EGFR activation occurred only in the presence of exogenous EGF. Although not statistically significant, increased density of Ki67 staining in the epithelium adjacent to the scratch wound was observed after treatment with EGF, suggesting a tendency for exogenous EGF to increase epithelial cell proliferation.
conclusionsExogenous EGF increases the rate of wound healing in an EGFR-dependent manner in a three-dimensional stem cell-derived model of vocal fold mucosa. This model of wound healing can be used to gain insight into the mechanisms that regulate vocal fold epithelial repair after injury.
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