ArticleBiomaterials2015
Ex vivo non-invasive assessment of cell viability and proliferation in bio-engineered whole organ constructs.
Article in Biomaterials, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed.
- Vascular Endothelial Growth Factor-D Improves Lung Vascular Integrity During Acute Lung Injury.Circulation research · 2026Article
- Mechanisms of nebivolol-mediated effects on bFGF-induced vascular smooth muscle cell proliferation and migration.Current research in pharmacology and drug discovery · 2025Article
- Heparin Immobilization Enhances Hemocompatibility, Re-Endothelization, and Angiogenesis of Decellularized Liver Scaffolds.International journal of molecular sciences · 2024Article
- Cardiac Tissue Engineering: A Journey from Scaffold Fabrication to In Vitro Characterization.Small science · 2024Article
- Breast Cancer Tissue Explants: An Approach to Develop Personalized Therapy in Public Health Services.Journal of personalized medicine · 2023Article
- Challenges of aortic valve tissue culture - maintenance of viability and extracellular matrix in the pulsatile dynamic microphysiological system.Journal of biological engineering · 2023Article
- Protease inhibitor Camostat Mesyalte blocks wild type SARS-CoV-2 and D614G viral entry in human engineered miniature lungs.Biomaterials · 2022Article
- Printer center nanoparticles alter the DNA repair capacity of human bronchial airway epithelial cells.NanoImpact · 2022Article
- Regulation of bFGF-induced effects on rat aortic smooth muscle cells by βCurrent research in pharmacology and drug discovery · 2022Article
- Human-scale lung regeneration based on decellularized matrix scaffolds as a biologic platform.Surgery today · 2020Review
- Lung Microvascular Niche, Repair, and Engineering.Frontiers in bioengineering and biotechnology · 2020Review
- A Fully Automated High-Throughput Bioreactor System for Lung Regeneration.Tissue engineering. Part C, Methods · 2018Article
- Improving functional re-endothelialization of acellular liver scaffold using REDV cell-binding domain.Acta biomaterialia · 2018Article
- Essential design considerations for the resazurin reduction assay to noninvasively quantify cell expansion within perfused extracellular matrix scaffolds.Biomaterials · 2017Article
- Advances in the Knowledge about Kidney Decellularization and Repopulation.Frontiers in bioengineering and biotechnology · 2017Review
- Regenerative potential of human airway stem cells in lung epithelial engineering.Biomaterials · 2016Article
- Enhanced Re-Endothelialization of Decellularized Rat Lungs.Tissue engineering. Part C, Methods · 2016Article
- Revascularization of decellularized lung scaffolds: principles and progress.American journal of physiology. Lung cellular and molecular physiology · 2015Review
- Engineering pulmonary vasculature in decellularized rat and human lungs.Nature biotechnology · 2015Article
- Dual-Purpose Bioreactors to Monitor Noninvasive Physical and Biochemical Markers of Kidney and Liver Scaffold Recellularization.Tissue engineering. Part C, Methods · 2015Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Decellularized organ scaffolds allow whole organ regeneration and study of cell behavior in three-dimensional culture conditions. Cell viability within the bio-engineered organ constructs is an essential parameter reflecting the performance of participating cells during long-term ex vivo culture, and is a prerequisite for further functional performance. Resazurin-based redox metabolic assays have been used to monitor cell viability in both two- and three-dimensional cell cultures. Here we developed a method for monitoring cell viability and proliferation in bio-engineered organ constructs using a resazurin perfusion assay. This method allows non-invasive, repetitive and rapid estimation of viable cell numbers during long-term ex vivo culture. As a proof-of-principle, we assessed the performance of two different endothelial sources and the impact of different perfusion programs on endothelial viability after re-endothelialization of decellularized lung scaffolds. The resazurin-based perfusion assay revealed changes in endothelial viability and proliferation during long-term ex vivo culture, which was consistent with histological assessment at different time points. Finally, we showed that this method could be used for assessment of proliferation and cytotoxicity after pharmacological treatment on a three-dimensional non-small cell lung cancer culture model.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.