ArticleCell reports2015
Genome engineering with CRISPR-Cas9 in the mosquito Aedes aegypti.
Article in Cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 191 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
191 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The ethics of genome editing in non-human animals: a systematic review of reasons reported in the academic literature.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Pooled it
- Mosquito-based malaria control through genetic, microbial and immunological innovations.Nature reviews. Microbiology · 2026Review
- Split suppression gene drives reveal a design tradeoff between inheritance and reproductive load inbioRxiv : the preprint server for biology · 2026Article
- Sweet Fennel Essential Oil RepelsInsects · 2026Article
- Odorant receptor coexpression and multi-expression in the dengue mosquito.Nature communications · 2026Article
- Advances in mosquito olfaction: Genetic, genomic, and behavioral approaches in Aedes aegypti.Current opinion in neurobiology · 2026Review
- A Molecular and Cellular Mechanism for Bitter Taste in the mosquitobioRxiv : the preprint server for biology · 2026Article
- Integrating multiplexing into confineable gene drives effectively overrides resistance in Anopheles stephensi.Nature communications · 2026Article
- A signaling hub in the mosquito rectum coordinates reproductive investment after blood feeding.Current biology : CB · 2026Article
- Neoclassical development of genetic sexing strains for insect pest and disease vector control.Insect science · 2026Review
- Pickpocket315 affects male mating behavior in the yellow fever mosquito Aedes aegypti.G3 (Bethesda, Md.) · 2026Article
- A genome-wide CRISPR screen in Anopheles mosquito cells identifies fitness and immune cell function-related genes.Nature communications · 2025Article
- Time-of-day modulation in mosquito response persistence to carbon dioxide is controlled by Pigment-Dispersing Factor.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A Signaling Hub in the Mosquito Rectum Coordinates Reproductive Investment After Blood Feeding.bioRxiv : the preprint server for biology · 2025Article
- The cycle gene is essential for both daily responses and seasonal reproduction in the Northern house mosquito, Culex pipiens.Scientific reports · 2025Article
- Article
- CRISPR/Cas9-Mediated Knockout of theInsects · 2025Article
- Transferable approaches to CRISPR-Cas9 induced genome editing in non-model insects: a brief guide.Frontiers in zoology · 2025Review
- Refined CRISPR/Cas9 genome editing in the pea aphid uncovers the essential roles of Laccase2 in overwintering egg adaptation.PLoS genetics · 2025Article
- Spider Eye Development Editing and Silk Fiber Engineering Using CRISPR-Cas.Angewandte Chemie (International ed. in English) · 2025Article
131 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
The mosquito Aedes aegypti is a potent vector of the chikungunya, yellow fever, and dengue viruses, responsible for hundreds of millions of infections and over 50,000 human deaths per year. Mutagenesis in Ae. aegypti has been established with TALENs, ZFNs, and homing endonucleases, which require the engineering of DNA-binding protein domains to provide genomic target sequence specificity. Here, we describe the use of the CRISPR-Cas9 system to generate site-specific mutations in Ae. aegypti. This system relies on RNA-DNA base-pairing to generate targeting specificity, resulting in efficient and flexible genome-editing reagents. We investigate the efficiency of injection mix compositions, demonstrate the ability of CRISPR-Cas9 to generate different types of mutations via disparate repair mechanisms, and report stable germline mutations in several genomic loci. This work offers a detailed exploration into the use of CRISPR-Cas9 in Ae. aegypti that should be applicable to non-model organisms previously out of reach of genetic modification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.