ArticleJournal of biomechanics2015
Engineering meniscus structure and function via multi-layered mesenchymal stem cell-seeded nanofibrous scaffolds.
Article in Journal of biomechanics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.
- Large Animal Models of Meniscus Repair and Regeneration: A Systematic Review of the State of the Field.Tissue engineering. Part C, Methods · 2017Pooled it
- Macroscale and microscale mechanical and biochemical properties of the equine superficial digital flexor tendon are regionally dependent.American journal of veterinary research · 2026Article
- Pneumatospinning and Electrospinning Scaffolds for Meniscus Regeneration Using Human Embryonic-Derived Mesenchymal Stem Cells.Bioengineering (Basel, Switzerland) · 2026Article
- Bioassembly of Region-Specific Fibrocartilage Microtissues to Engineer Zonally Defined Meniscal Grafts.Advanced healthcare materials · 2025Article
- Controlled Stiffness of Direct-Write, Near-Field Electrospun Gelatin Fibers Generates Differences in Tenocyte Morphology and Gene Expression.Journal of biomechanical engineering · 2024Article
- Development of 3D Printable Gelatin Methacryloyl/Chondroitin Sulfate/Hyaluronic Acid Hydrogels as Implantable Scaffolds.Polymers · 2024Article
- An Update on Applications of Cattle Mesenchymal Stromal Cells.Animals : an open access journal from MDPI · 2022Review
- Prospective Application of Partially Digested Autologous Chondrocyte for Meniscus Tissue Engineering.Pharmaceutics · 2022Article
- Pneumatospinning Biomimetic Scaffolds for Meniscus Tissue Engineering.Frontiers in bioengineering and biotechnology · 2022Article
- Biomaterials and Meniscal Lesions: Current Concepts and Future Perspective.Pharmaceutics · 2021Review
- Quantitative three-dimensional collagen orientation analysis of human meniscus posterior horn in health and osteoarthritis using micro-computed tomography.Osteoarthritis and cartilage · 2021Article
- Meniscal allograft transplants and new scaffolding techniques.EFORT open reviews · 2019Review
- TRPV4-mediated calcium signaling in mesenchymal stem cells regulates aligned collagen matrix formation and vinculin tension.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Current Concepts in Meniscus Tissue Engineering and Repair.Advanced healthcare materials · 2018Review
- Meniscal Tissue Engineering Using Aligned Collagen Fibrous Scaffolds: Comparison of Different Human Cell Sources.Tissue engineering. Part A · 2018Article
- A review of in-vitro fibrocartilage tissue engineered therapies with a focus on the temporomandibular joint.Archives of oral biology · 2017Review
- Article
- Micromechanical anisotropy and heterogeneity of the meniscus extracellular matrix.Acta biomaterialia · 2017Article
- Braided and Stacked Electrospun Nanofibrous Scaffolds for Tendon and Ligament Tissue Engineering.Tissue engineering. Part A · 2017Article
- Anatomical region-dependent enhancement of 3-dimensional chondrogenic differentiation of human mesenchymal stem cells by soluble meniscus extracellular matrix.Acta biomaterialia · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Despite advances in tissue engineering for the knee meniscus, it remains a challenge to match the complex macroscopic and microscopic structural features of native tissue, including the circumferentially and radially aligned collagen bundles essential for mechanical function. To mimic this structural hierarchy, this study developed multi-lamellar mesenchymal stem cell (MSC)-seeded nanofibrous constructs. Bovine MSCs were seeded onto nanofibrous scaffolds comprised of poly(ε-caprolactone) with fibers aligned in a single direction (0° or 90° to the scaffold long axis) or circumferentially aligned (C). Multi-layer groups (0°/0°/0°, 90°/90°/90°, 0°/90°/0°, 90°/0°/90°, and C/C/C) were created and cultured for a total of 6 weeks under conditions favoring fibrocartilaginous tissue formation. Tensile testing showed that 0° and C single layer constructs had stiffness values several fold higher than 90° constructs. For multi-layer groups, the stiffness of 0°/0°/0° constructs was higher than all other groups, while 90°/90°/90° constructs had the lowest values. Data for collagen content showed a general positive interactive effect for multi-layers relative to single layer constructs, while a positive interaction for stiffness was found only for the C/C/C group. Collagen content and cell infiltration occurred independent of scaffold alignment, and newly formed collagenous matrix followed the scaffold fiber direction. Structural hierarchies within multi-lamellar constructs dictated biomechanical properties, and only the C/C/C constructs with non-orthogonal alignment within layers featured positive mechanical reinforcement as a consequence of the layered construction. These multi-layer constructs may serve as functional substitutes for the meniscus as well as test beds to understand the complex mechanical principles that enable meniscus function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.