ArticleJournal of molecular biology2015
Key residues of S. flexneri OmpA mediate infection by bacteriophage Sf6.
Article in Journal of molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- TheJournal of virology · 2025Article
- Moo19 and B2: Structures ofScience advances · 2024Article
- Comparative Genomics of Six LyticPHAGE (New Rochelle, N.Y.) · 2022Article
- Host Range Expansion ofJournal of virology · 2022Article
- Biological foundations of successful bacteriophage therapy.EMBO molecular medicine · 2022Review
- Shigella viruses Sf22 and KRT47 require outer membrane protein C for infection.Biochimica et biophysica acta. Biomembranes · 2022Article
- Mosaic Evolution of Beta-Barrel-Porin-Encoding Genes inApplied and environmental microbiology · 2022Article
- Isolation and characterization of lytic phage TUN1 specific for Klebsiella pneumoniae K64 clinical isolates from Tunisia.BMC microbiology · 2021Article
- Diversity and characterization of temperate bacteriophages induced in Pasteurella multocida from different host species.BMC microbiology · 2021Article
- Ecology, Structure, and Evolution ofAnnual review of virology · 2020Review
- Screening of Polyvalent Phage-ResistantFrontiers in microbiology · 2019Article
- A Smooth-Type, Phage-Resistant Klebsiella pneumoniae Mutant Strain Reveals that OmpC Is Indispensable for Infection by Phage GH-K3.Applied and environmental microbiology · 2018Article
- Gain-of-function experiments with bacteriophage lambda uncover residues under diversifying selection in nature.Evolution; international journal of organic evolution · 2018Article
- Bacteriophage Sf6 Tailspike Protein for Detection ofViruses · 2018Article
- Genes affecting progression of bacteriophage P22 infection in Salmonella identified by transposon and single gene deletion screens.Molecular microbiology · 2018Article
- The outer-membrane protein TolC ofThe Journal of biological chemistry · 2018Article
- Evolved Populations of Shigella flexneri Phage Sf6 Acquire Large Deletions, Altered Genomic Architecture, and Faster Life Cycles.Genome biology and evolution · 2016Article
- Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Many viruses, including bacteriophage, have the inherent ability to utilize several types of proteinaceous receptors as an attachment mechanism to infect cells, yet the molecular mechanisms that drive receptor binding have not been elucidated. Using bacteriophage Sf6 and its host, Shigella flexneri, we investigated how Sf6 utilizes outer membrane protein A (OmpA) for infection. Specifically, we identified that surface loops of OmpA mediate Shigella infection. We further characterized which residues in the surface loops are responsible for Sf6 binding and productive infection using a combination of in vivo and in vitro approaches including site-directed mutagenesis, phage plaque assays, circular dichroism spectroscopy, and in vitro genome ejection assays. Our data indicate that Sf6 can productively interact with other bacterial OmpAs as long as they share homology in loops 2 and 4, suggesting that these loops may determine host specificity. Our data provide a model in which Sf6 interacts with OmpA using the surface of the protein and new insights into viral attachment through binding to membrane protein receptors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.