ArticleOncotarget2015
Quantification of cellular viability by automated microscopy and flow cytometry.
Article in Oncotarget, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed.
- Simultaneous measurement of target and effector cell death, apoptosis, and proliferation in cell therapy development using flow cytometry.STAR protocols · 2026Article
- In mice, a population of male germ cells show characteristics of non-apoptotic cell death during G0 arrest.bioRxiv : the preprint server for biology · 2026Article
- Stable charged nanobubbles with distinct polarities in culture media differentially affect the viability of human iPSC-derived neurons.Scientific reports · 2026Article
- Mitochondrial Adaptations Underlying Tetraploidization in Human Cancer, Fungal, and Yeast Models.Biology · 2026Article
- Evaluating cell viability assessment techniques: a comparative study of flow cytometry and fluorescence microscopy in response to bioactive glass exposure.Biomedical engineering online · 2025Article
- Review
- Pathogenic role of acyl coenzyme A binding protein (ACBP) in Cushing's syndrome.Nature metabolism · 2024Article
- Complementary Approaches to Interrogate Mitophagy Flux in Pancreatic β-Cells.Journal of visualized experiments : JoVE · 2023Article
- Article
- Glutamine deprivation induces ferroptosis in pancreatic cancer cells.Acta biochimica et biophysica Sinica · 2023Article
- Integration of Single-Cell Transcriptomics With a High Throughput Functional Screening Assay to Resolve Cell Type, Growth Kinetics, and Stemness Heterogeneity Within the Comma-1D Cell Line.Frontiers in genetics · 2022Article
- IGF1 receptor inhibition amplifies the effects of cancer drugs by autophagy and immune-dependent mechanisms.Journal for immunotherapy of cancer · 2021Article
- Control of stress-induced apoptosis by freezing tolerance-associated wheat proteins during cryopreservation of rat hepatocytes.Cell stress & chaperones · 2020Article
- Cell Clustering Promotes a Metabolic Switch that Supports Metastatic Colonization.Cell metabolism · 2019Article
- A Flow Cytometry-Based Assay for High-Throughput Detection and Quantification of Neutrophil Extracellular Traps in Mixed Cell Populations.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
- Mitophagy Failure in Fibroblasts and iPSC-Derived Neurons of Alzheimer's Disease-Associated Presenilin 1 Mutation.Frontiers in molecular neuroscience · 2017Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cellular viability is usually determined by measuring the capacity of cells to exclude vital dyes such as 4',6-diamidino-2-phenylindole (DAPI), or by assessing nuclear morphology with chromatinophilic plasma membrane-permeant dyes, such as Hoechst 33342. However, a fraction of cells that exclude DAPI or exhibit normal nuclear morphology have already lost mitochondrial functions and/or manifest massive activation of apoptotic caspases, and hence are irremediably committed to death. Here, we developed a protocol for the simultaneous detection of plasma membrane integrity (based on DAPI) or nuclear morphology (based on Hoechst 33342), mitochondrial functions (based on the mitochondrial transmembrane potential probe DiOC6(3)) and caspase activation (based on YO-PRO®-3, which can enter cells exclusively upon the caspase-mediated activation of pannexin 1 channels). This method, which allows for the precise quantification of dead, dying and healthy cells, can be implemented on epifluorescence microscopy or flow cytometry platforms and is compatible with a robotized, high-throughput workflow.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.