ArticleGeneral physiology and biophysics2015
System for exposing cultured cells to intermittent hypoxia utilizing gas permeable cultureware.
Article in General physiology and biophysics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Continuous flow delivery system for the perfusion of scaffold-based 3D cultures.Lab on a chip · 2024Article
- Assessing MTT and sulforhodamine B cell proliferation assays under multiple oxygen environments.Cytotechnology · 2023Article
- CORP: Sources and degrees of variability in whole animal intermittent hypoxia experiments.Journal of applied physiology (Bethesda, Md. : 1985) · 2023Article
- Intermittent hypoxia treatments cause cellular priming in human microglia.Journal of cellular and molecular medicine · 2023Article
- Experimental Models to Study End-Organ Morbidity in Sleep Apnea: Lessons Learned and Future Directions.International journal of molecular sciences · 2022Review
- Hypoxia-Induced Sarcoplasmic Reticulum Ca2+ Leak Is Reversed by Ryanodine Receptor Stabilizer JTV-519 in HL-1 Cardiomyocytes.Anatolian journal of cardiology · 2022Article
- Hypoxia Induces Saturated Fatty Acids Accumulation and Reduces Unsaturated Fatty Acids Independently of Reverse Tricarboxylic Acid Cycle in L6 Myotubes.Frontiers in endocrinology · 2022Article
- Experimental Modeling of Damaging and Protective Hypoxia of the Mammalian Brain.Journal of evolutionary biochemistry and physiology · 2022Article
- A multiwell plate-based system for toxicity screening under multiple static or cycling oxygen environments.Scientific reports · 2021Article
- Technical Feasibility and Physiological Relevance of Hypoxic Cell Culture Models.Frontiers in endocrinology · 2020Review
- Flow-Encoded Oxygen Control to Track the Time-Dependence of Molecular Changes Induced by Static or Cycling Hypoxia.Analytical chemistry · 2019Article
- Hypoxia Modulates Effects of Fatty Acids on NES2Y Human Pancreatic β-cells.International journal of molecular sciences · 2019Article
- Chronic intermittent hypoxia enhances disease progression in myeloma-resistant mice.American journal of physiology. Regulatory, integrative and comparative physiology · 2019Article
- Mixing and delivery of multiple controlled oxygen environments to a single multiwell culture plate.American journal of physiology. Cell physiology · 2018Article
- The Effect of Hypoxia and Metformin on Fatty Acid Uptake, Storage, and Oxidation in L6 Differentiated Myotubes.Frontiers in endocrinology · 2018Article
- Novel in vivo and in vitro techniques to image and model the cerebral vasculature in sickle cell disease.Blood cells, molecules & diseases · 2017Review
- A Novel Chip for Cyclic Stretch and Intermittent Hypoxia Cell Exposures Mimicking Obstructive Sleep Apnea.Frontiers in physiology · 2016Article
- Article
- Frequently asked questions in hypoxia research.Hypoxia (Auckland, N.Z.) · 2015Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Tissue intermittent hypoxia (IH) occurs in obstructive sleep apnea, sickle cell anemia, physical exercise and other conditions. Poor gas solubility and slow diffusion through culture media hampers mimicking IH-induced transitions of O(2) in vitro. We aimed to develop a system enabling exposure of cultured cells to IH and to validate such exposure by real-time O(2) measurements and cellular responses. Standard 24-well culture plates and plates with bottoms made from a gas permeable film were placed in a heated cabinet. Desired cycling of O(2) levels was induced using programmable solenoids to purge mixtures of 95% N(2) + 5% CO(2) or 95% O(2) + 5% CO(2). Dissolved oxygen, gas pressure, temperature, and water evaporation were measured during cycling. IH-induced cellular effects were evaluated by hypoxia inducible factor (HIF) and NF-κB luciferase reporters in HEK296 cells and by insulin secretion in rat insulinoma cells. Oxygen cycling in the cabinet was translated into identical changes of O(2) at the well bottom in gas permeable, but not in standard cultureware. Twenty-four hours of IH exposure increased HIF (112%), NF-κB (111%) and insulin secretion (44%). Described system enables reproducible and prolonged IH exposure in cultured cells while controlling for important environmental factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.