ArticlePloS one2015
Assessing glucose uptake through the yeast hexose transporter 1 (Hxt1).
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
- Selective targeting ofAntimicrobial agents and chemotherapy · 2026Article
- Refining the resolution of the yeast genotype-phenotype map using single-cell RNA-sequencing.eLife · 2025Article
- Modulation of transmembrane domain architecture by the intracellular C-terminal domain in yeast glucose transporter-receptor divergence.Frontiers in molecular biosciences · 2025Review
- Multiple roles for the cytoplasmic C-terminal domains of the yeast cell surface receptors Rgt2 and Snf3 in glucose sensing and signaling.Scientific reports · 2024Article
- Structural and biochemical insights of xylose MFS and SWEET transporters in microbial cell factories: challenges to lignocellulosic hydrolysates fermentation.Frontiers in microbiology · 2024Review
- The role of salt bridge networks in the stability of the yeast hexose transporter 1.Biochimica et biophysica acta. General subjects · 2023Article
- Functional Analysis of Plant Monosaccharide Transporters Using a Simple Growth Complementation Assay in Yeast.Bio-protocol · 2023Article
- Temporal segregation of biosynthetic processes is responsible for metabolic oscillations during the budding yeast cell cycle.Nature metabolism · 2023Article
- Genomic evolution towards azole resistance in Candida glabrata clinical isolates unveils the importance of CgHxt4/6/7 in azole accumulation.Communications biology · 2022Article
- 2-deoxyglucose transiently inhibits yeast AMPK signaling and triggers glucose transporter endocytosis, potentiating the drug toxicity.PLoS genetics · 2022Article
- Article
- The yapsin family of aspartyl proteases regulate glucose homeostasis in Candida glabrata.The Journal of biological chemistry · 2022Article
- Identification of key residues for efficient glucose transport by the hexose transporter CgHxt4 in high sugar fermentation yeast Candida glycerinogenes.Applied microbiology and biotechnology · 2021Article
- Role of Calcium/Calcineurin Signalling in Regulating Intracellular Reactive Oxygen Species Homeostasis inGenes · 2021Article
- Crossing the blood-brain barrier with carbon dots: uptake mechanism andNanoscale advances · 2021Article
- A label-free real-time method for measuring glucose uptake kinetics in yeast.FEMS yeast research · 2021Article
- Regulation and metabolic engineering strategies for permeases of Saccharomyces cerevisiae.World journal of microbiology & biotechnology · 2019Review
- The Plastidic Sugar Transporter pSuT Influences Flowering and Affects Cold Responses.Plant physiology · 2019Article
- An artificial transport metabolon facilitates improved substrate utilization in yeast.Nature chemical biology · 2017Article
- Multilevel regulation of an α-arrestin by glucose depletion controls hexose transporter endocytosis.The Journal of cell biology · 2017Article
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Authors and funding
4 authors at 3 institutions in 1 country.
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Abstract
The transport of glucose across the plasma membrane is mediated by members of the glucose transporter family. In this study, we investigated glucose uptake through the yeast hexose transporter 1 (Hxt1) by measuring incorporation of 2-NBDG, a non-metabolizable, fluorescent glucose analog, into the yeast Saccharomyces cerevisiae. We find that 2-NBDG is not incorporated into the hxt null strain lacking all glucose transporter genes and that this defect is rescued by expression of wild type Hxt1, but not of Hxt1 with mutations at the putative glucose-binding residues, inferred from the alignment of yeast and human glucose transporter sequences. Similarly, the growth defect of the hxt null strain on glucose is fully complemented by expression of wild type Hxt1, but not of the mutant Hxt1 proteins. Thus, 2-NBDG, like glucose, is likely to be transported into the yeast cells through the glucose transport system. Hxt1 is internalized and targeted to the vacuole for degradation in response to glucose starvation. Among the mutant Hxt1 proteins, Hxt1N370A and HXT1W473A are resistant to such degradation. Hxt1N370A, in particular, is able to neither uptake 2-NBDG nor restore the growth defect of the hxt null strain on glucose. These results demonstrate 2-NBDG as a fluorescent probe for glucose uptake in the yeast cells and identify N370 as a critical residue for the stability and function of Hxt1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.