ArticleMedicine2015
Medium-based noninvasive preimplantation genetic diagnosis for human α-thalassemias-SEA.
Article in Medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.
- Evaluation of non-invasive gene detection in preimplantation embryos: a systematic review and meta-analysis.Journal of assisted reproduction and genetics · 2023Pooled it
- "Old and new" frontiers in embryo viability assessment: current practices and introduction of emerging approaches.Biology of reproduction · 2026Review
- Non-invasive embryo assessment: Cell-free DNA-based genetic testing and amino acid metabolomics in relation to morphology: A case-control study.International journal of reproductive biomedicine · 2026Article
- Application of non-invasive preimplantation genetic screening for aneuploidy through spent embryo culture media analysis at 48 and 54 hours after embryo cleavage.British journal of biomedical science · 2026Article
- Review
- Non-Invasive Preimplantation Genetic Testing: Cell-Free DNA Detection in Embryo Culture Media Using a Plasmonic Biosensor.Analytical chemistry · 2025Article
- Non-Invasive Preimplantation Genetic Testing.Genes · 2025Review
- A computational DNA methylation method to remove contaminated DNA from spent embryo culture medium for noninvasive preimplantation genetic testing.EBioMedicine · 2025Article
- Reproductive Choices in Haemoglobinopathies: The Role of Preimplantation Genetic Testing.Genes · 2025Review
- Review
- Evolution of Minimally Invasive and Non-Invasive Preimplantation Genetic Testing: An Overview.Journal of clinical medicine · 2024Review
- Comparing the advantages, disadvantages and diagnostic power of different non-invasive pre-implantation genetic testing: A literature review.International journal of reproductive biomedicine · 2023Review
- Non-invasive evaluation of embryo quality for the selection of transferable embryos in human in vitro fertilization-embryo transfer.Clinical and experimental reproductive medicine · 2022Article
- An Update on Non-invasive Approaches for Genetic Testing of the Preimplantation Embryo.Current genomics · 2022Review
- Majority of transferred mosaic embryos developed healthy live births revealed by a preclinical study using embryonic morphology assessment and noninvasive PGT-A on cell-free DNA in blastocoel fluid.Journal of assisted reproduction and genetics · 2022Article
- Non-invasive preimplantation genetic testing: a literature review.JBRA assisted reproduction · 2022Review
- A prospective study to evaluate the gender prediction of blastocysts by using cell-free DNA within a culture medium.International journal of reproductive biomedicine · 2022Article
- Liquid biopsy: state of reproductive medicine and beyond.Human reproduction (Oxford, England) · 2021Review
- NGS-Based Application for Routine Non-Invasive Pre-Implantation Genetic Assessment in IVF.International journal of molecular sciences · 2021Article
- Improved Non-Invasive Preimplantation Genetic Testing for Beta-Thalassemia Using Spent Embryo Culture Medium Containing Blastocoelic Fluid.Frontiers in endocrinology · 2021Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To develop a noninvasive medium-based preimplantation genetic diagnosis (PGD) test for α-thalassemias-SEA. The embryos of α-thalassemia-SEA carriers undergoing in vitro fertilization (IVF) were cultured. Single cells were biopsied from blastomeres and subjected to fluorescent gap polymerase chain reaction (PCR) analysis; the spent culture media that contained embryo genomic DNA and corresponding blastocysts as verification were subjected to quantitative-PCR (Q-PCR) detection of α-thalassemia-SEA. The diagnosis efficiency and allele dropout (ADO) ratio were calculated, and the cell-free DNA concentration was quantitatively assessed in the culture medium. The diagnosis efficiency of medium-based α-thalassemias-SEA detection significantly increased compared with that of biopsy-based fluorescent gap PCR analysis (88.6% vs 82.1%, P < 0.05). There is no significant difference regarding ADO ratio between them. The optimal time for medium-based α-thalassemias-SEA detection is Day 5 (D5) following IVF. Medium-based α-thalassemias-SEA detection could represent a novel, quick, and noninvasive approach for carriers to undergo IVF and PGD.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.