ArticlePLoS pathogens2015
Exome and transcriptome sequencing of Aedes aegypti identifies a locus that confers resistance to Brugia malayi and alters the immune response.
Article in PLoS pathogens, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- Wolbachia infection in Aedes aegypti does not affect its vectorial capacity for Dirofilaria immitis.Scientific reports · 2024Article
- Extracellular vesicles secreted by Brugia malayi microfilariae modulate the melanization pathway in the mosquito host.Scientific reports · 2023Article
- Differences in gene expression in field populations of Wolbachia-infected Aedes aegypti mosquitoes with varying release histories in northern Australia.PLoS neglected tropical diseases · 2023Article
- Aedes aegypti Malpighian tubules are immunologically activated following systemic Toll activation.Parasites & vectors · 2022Article
- Permethrin resistance in Aedes aegypti: Genomic variants that confer knockdown resistance, recovery, and death.PLoS genetics · 2021Article
- A Two-Locus System with Strong Epistasis Underlies Rapid Parasite-Mediated Evolution of Host Resistance.Molecular biology and evolution · 2021Article
- The mosquito protein AEG12 displays both cytolytic and antiviral properties via a common lipid transfer mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Review
- Recent advances and future perspectives in vector-omics.Current opinion in insect science · 2020Review
- Exome-wide association study reveals largely distinct gene sets underlying specific resistance to dengue virus types 1 and 3 in Aedes aegypti.PLoS genetics · 2020Article
- Activation of mosquito immunity blocks the development of transmission-stage filarial nematodes.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Exome-wide association of deltamethrin resistance in Aedes aegypti from Mexico.Insect molecular biology · 2019Article
- Pathogen blocking in Wolbachia-infected Aedes aegypti is not affected by Zika and dengue virus co-infection.PLoS neglected tropical diseases · 2019Article
- Vgsc-interacting proteins are genetically associated with pyrethroid resistance in Aedes aegypti.PloS one · 2019Article
- Ethanol as a potential mosquito sample storage medium for RNA preservation.F1000Research · 2019Article
- Article
- Mosquitoes and the Lymphatic Filarial Parasites: Research Trends and Budding Roadmaps to Future Disease Eradication.Tropical medicine and infectious disease · 2018Review
- Alternative patterns of sex chromosome differentiation in Aedes aegypti (L).BMC genomics · 2017Article
- New Insights into Cockroach Allergens.Current allergy and asthma reports · 2017Review
- Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
Many mosquito species are naturally polymorphic for their abilities to transmit parasites, a feature which is of great interest for controlling vector-borne disease. Aedes aegypti, the primary vector of dengue and yellow fever and a laboratory model for studying lymphatic filariasis, is genetically variable for its capacity to harbor the filarial nematode Brugia malayi. The genome of Ae. aegypti is large and repetitive, making genome resequencing difficult and expensive. We designed exome captures to target protein-coding regions of the genome, and used association mapping in a wild Kenyan population to identify a single, dominant, sex-linked locus underlying resistance. This falls in a region of the genome where a resistance locus was previously mapped in a line established in 1936, suggesting that this polymorphism has been maintained in the wild for the at least 80 years. We then crossed resistant and susceptible mosquitoes to place both alleles of the gene into a common genetic background, and used RNA-seq to measure the effect of this locus on gene expression. We found evidence for Toll, IMD, and JAK-STAT pathway activity in response to early stages of B. malayi infection when the parasites are beginning to die in the resistant genotype. We also found that resistant mosquitoes express anti-microbial peptides at the time of parasite-killing, and that this expression is suppressed in susceptible mosquitoes. Together, we have found that a single resistance locus leads to a higher immune response in resistant mosquitoes, and we identify genes in this region that may be responsible for this trait.
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Registered trials
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