ArticlePloS one2015
Heritable CRISPR/Cas9-mediated genome editing in the yellow fever mosquito, Aedes aegypti.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 125 citations in OpenAlex.
- Plant resistance: scientific basis and latest research progress.Frontiers in plant science · 2026Review
- Transferable approaches to CRISPR-Cas9 induced genome editing in non-model insects: a brief guide.Frontiers in zoology · 2025Review
- Spider Eye Development Editing and Silk Fiber Engineering Using CRISPR-Cas.Angewandte Chemie (International ed. in English) · 2025Article
- History of Mosquito Transgenesis: A Perspective in Review.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Manipulation of a New Non-model Insect Genome Using Targeted CRISPR-Era Approaches.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Direct Parental (DIPA) CRISPR in the jewel wasp, Nasonia vitripennis.G3 (Bethesda, Md.) · 2024Article
- The Development and Expansion of in vivo Germline Editing Technologies in Arthropods: Receptor-Mediated Ovary Transduction of Cargo (ReMOT Control) and Beyond.Integrative and comparative biology · 2023Review
- CRISPR-Cas Technology as a Revolutionary Genome Editing tool: Mechanisms and Biomedical Applications.Iranian biomedical journal · 2023Review
- Article
- CRISPR-Cas9 Genome Editing Uncovers the Mode of Action of Methoprene in the Yellow Fever Mosquito,The CRISPR journal · 2022Article
- Cas9-mediated gene editing in the black-legged tick,iScience · 2022Article
- Article
- Expanding the Toolkit for Genome Editing in a Disease Vector,The CRISPR journal · 2021Article
- Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences.Biotech (Basel (Switzerland)) · 2021Review
- Suppression of female fertility inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines.Scientific reports · 2021Article
- How to turn an organism into a model organism in 10 'easy' steps.The Journal of experimental biology · 2020Review
- Review
- Leaning Into the Bite: The piRNA Pathway as an Exemplar for the Genetic Engineering Need in Mosquitoes.Frontiers in cellular and infection microbiology · 2020Article
- Knockout of juvenile hormone receptor, Methoprene-tolerant, induces black larval phenotype in the yellow fever mosquito,Proceedings of the National Academy of Sciences of the United States of America · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
In vivo targeted gene disruption is a powerful tool to study gene function. Thus far, two tools for genome editing in Aedes aegypti have been applied, zinc-finger nucleases (ZFN) and transcription activator-like effector nucleases (TALEN). As a promising alternative to ZFN and TALEN, which are difficult to produce and validate using standard molecular biological techniques, the clustered regularly interspaced short palindromic repeats/CRISPR-associated sequence 9 (CRISPR/Cas9) system has recently been discovered as a "do-it-yourself" genome editing tool. Here, we describe the use of CRISPR/Cas9 in the mosquito vector, Aedes aegypti. In a transgenic mosquito line expressing both Dsred and enhanced cyan fluorescent protein (ECFP) from the eye tissue-specific 3xP3 promoter in separated but tightly linked expression cassettes, we targeted the ECFP nucleotide sequence for disruption. When supplying the Cas9 enzyme and two sgRNAs targeting different regions of the ECFP gene as in vitro transcribed mRNAs for germline transformation, we recovered four different G1 pools (5.5% knockout efficiency) where individuals still expressed DsRed but no longer ECFP. PCR amplification, cloning, and sequencing of PCR amplicons revealed indels in the ECFP target gene ranging from 2-27 nucleotides. These results show for the first time that CRISPR/Cas9 mediated gene editing is achievable in Ae. aegypti, paving the way for further functional genomics related studies in this mosquito species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.