Evidence map›Paper›PMID 25815330›Full record

ArticleBioMed research international2015

Ascorbic acid, ultraviolet C rays, and glucose but not hyperthermia are elicitors of human β-defensin 1 mRNA in normal keratinocytes.

Luis Antonio Cruz Díaz, María Guadalupe Flores Miramontes, Paulina Chávez Hurtado, Kirk Allen, Marisela Gonzalez Ávila, Ernesto Prado Montes de Oca

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BioMed research international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 20 citations in OpenAlex.

  1. Trial
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  9. The effect of Vitamin C on melanin pigmentation - A systematic review.Journal of oral and maxillofacial pathology : JOMFP
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 2 countries.

Luis Antonio Cruz DíazMolecular Biology Laboratory, Biosecurity Area, Pharmaceutical and Medical Biotechnology Unit (UBMF), Research Center in Technology and Design Assistance of Jalisco State (CIATEJ, AC), National Council of Science and Technology (CONACYT), Guadalajara, JAL, Mexico.
María Guadalupe Flores MiramontesMolecular Biology Laboratory, Biosecurity Area, Pharmaceutical and Medical Biotechnology Unit (UBMF), Research Center in Technology and Design Assistance of Jalisco State (CIATEJ, AC), National Council of Science and Technology (CONACYT), Guadalajara, JAL, Mexico.
Paulina Chávez HurtadoMolecular Biology Laboratory, Biosecurity Area, Pharmaceutical and Medical Biotechnology Unit (UBMF), Research Center in Technology and Design Assistance of Jalisco State (CIATEJ, AC), National Council of Science and Technology (CONACYT), Guadalajara, JAL, Mexico.ORCID 0000-0003-4613-3151
Kirk AllenIn silico Laboratory, UBMF, CIATEJ AC, CONACYT, Guadalajara, JAL, Mexico ; Lancaster Medical School, Lancaster University, Lancaster, UK.
Marisela Gonzalez ÁvilaHuman Digestive Tract Simulator, UBMF, CIATEJ, AC, CONACYT, Guadalajara, JAL, Mexico.
Ernesto Prado Montes de OcaMolecular Biology Laboratory, Biosecurity Area, Pharmaceutical and Medical Biotechnology Unit (UBMF), Research Center in Technology and Design Assistance of Jalisco State (CIATEJ, AC), National Council of Science and Technology (CONACYT), Guadalajara, JAL, Mexico ; In silico Laboratory, UBMF, CIATEJ AC, CONACYT, Guadalajara, JAL, Mexico.
Secretaría de Ciencia, Humanidades, Tecnología e Innovación · MX

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hosts' innate defense systems are upregulated by antimicrobial peptide elicitors (APEs). Our aim was to investigate the effects of hyperthermia, ultraviolet A rays (UVA), and ultraviolet C rays (UVC) as well as glucose and ascorbic acid (AA) on the regulation of human β-defensin 1 (DEFB1), cathelicidin (CAMP), and interferon-γ (IFNG) genes in normal human keratinocytes (NHK). The indirect in vitro antimicrobial activity against Staphylococcus aureus and Listeria monocytogenes of these potential APEs was tested. We found that AA is a more potent APE for DEFB1 than glucose in NHK. Glucose but not AA is an APE for CAMP. Mild hypo- (35°C) and hyperthermia (39°C) are not APEs in NHK. AA-dependent DEFB1 upregulation below 20 mM predicts in vitro antimicrobial activity as well as glucose- and AA-dependent CAMP and IFNG upregulation. UVC upregulates CAMP and DEFB1 genes but UVA only upregulates the DEFB1 gene. UVC is a previously unrecognized APE in human cells. Our results suggest that glucose upregulates CAMP in an IFN-γ-independent manner. AA is an elicitor of innate immunity that will challenge the current concept of late activation of adaptive immunity of this vitamin. These results could be useful in designing new potential drugs and devices to combat skin infections.

Indexed as

Ultraviolet RaysAntimicrobial Cationic PeptidesAscorbic Acidbeta-DefensinsCathelicidinsFeverGene Expression RegulationGlucoseHumansImmunity, InnateInterferon-gammaKeratinocytesListeria monocytogenesRNA, MessengerStaphylococcus aureusAntimicrobial Cationic PeptidesAscorbic Acidbeta-DefensinsCathelicidinsDEFB1 protein, humanGlucoseInterferon-gammaRNA, Messenger

Identifiers

PMID25815330
PMCPMC4359827
OpenAlexW2036504746

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.