ArticleInternational journal of genomics2015
Validation of simple sequence length polymorphism regions of commonly used mouse strains for marker assisted speed congenics screening.
Article in International journal of genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Genetic quality assurance and genetic monitoring of laboratory mice and rats: FELASA Working Group Report.Laboratory animals · 2020Guideline
- Generation of mouse and rat xenogeneic ovariesAnimal cells and systems · 2024Article
- The protective role of conjunctival goblet cell mucin sialylation.Nature communications · 2023Article
- A new mouse model of Ehlers-Danlos syndrome generated using CRISPR/Cas9-mediated genomic editing.Disease models & mechanisms · 2021Article
- A new mouse SNP genotyping assay for speed congenics: combining flexibility, affordability, and power.BMC genomics · 2021Article
- Designing and generating a mouse model: frequently asked questions.Journal of biomedical research · 2021Article
- Antiretroviral Drug Metabolism in Humanized PXR-CAR-CYP3A-NOG Mice.The Journal of pharmacology and experimental therapeutics · 2018Article
- Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors.Nature protocols · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Marker assisted speed congenics technique is commonly used to facilitate backcrossing of mouse strains in nearly half the time it normally takes otherwise. Traditionally, the technique is performed by analyzing PCR amplified regions of simple sequence length polymorphism (SSLP) markers between the recipient and donor strains: offspring with the highest number of markers showing the recipient genome across all chromosomes is chosen for the next generation. Although there are well-defined panels of SSLP makers established between certain pairs of mice strains, they are incomplete for most strains. The availability of well-established marker sets for speed congenic screens would enable the scientific community to transfer mutations across strain backgrounds. In this study, we tested the suitability of over 400 SSLP marker sets among 10 mouse strains commonly used for generating genetically engineered models. The panel of markers presented here can readily identify the specified strains and will be quite useful in marker assisted speed congenic screens. Moreover, unlike newer single nucleotide polymorphism (SNP) array methods which require sophisticated equipment, the SSLP markers panel described here only uses PCR and agarose gel electrophoresis of amplified products; therefore it can be performed in most research laboratories.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.