ArticleInternational journal of molecular and cellular medicine2015
RNA Extraction from Animal and Human's Cancerous Tissues: Does Tissue Matter?
Article in International journal of molecular and cellular medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- Optimizing RNA quality in cryopreserved tissues without preservatives: impact of preservatives, thawing methods and tissue aliquot sizes.BMC biotechnology · 2025Article
- Investigation of the Renal Defensive Influence of Walnut Septa Extract Against Acute Renal Ischemia/Reperfusion Injury.Mediators of inflammation · 2025Article
- Enhancing negative control selection: A comparative analysis of random and targeted sampling techniques for obtaining High-Quality RNA from normal breast tissue.Biology methods & protocols · 2024Article
- Cyanidin-3-Glucoside Modulates hsa_circ_0001345/miRNA106b/ATG16L1 Axis Expression as a Potential Protective Mechanism against Hepatocellular Carcinoma.Current issues in molecular biology · 2022Article
- Overcoming the Challenges of High Quality RNA Extraction from Core Needle Biopsy.Biomolecules · 2021Article
- Identification of an optimal method for extracting RNA from human skin biopsy, using domestic pig as a model system.Scientific reports · 2019Article
- Regulatory Fluctuation of WNT16 Gene Expression Is Associated with Human Gastric Adenocarcinoma.Journal of gastrointestinal cancer · 2019Article
- The Effects of Surgical Antiseptics and Time Delays on RNA Isolated From Human and Rodent Peripheral Nerves.Frontiers in cellular neuroscience · 2019Article
- Application of unique sequence index (USI) barcode to gene expression profiling in gastric adenocarcinoma.Journal of cell communication and signaling · 2017Article
- FAT4 hypermethylation and grade dependent downregulation in gastric adenocarcinoma.Journal of cell communication and signaling · 2017Article
- Immune cell profiling in cancer: molecular approaches to cell-specific identification.NPJ precision oncology · 2017Review
- Interaction of sonic hedgehog (SHH) pathway with cancer stem cell genes in gastric cancer.Medical oncology (Northwood, London, England) · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The reliability of gene expression profiling, based technologies and methods to find transcriptional differences representative of the original samples is influenced by the quality of the extracted RNA. Hence, RNA extraction is the first step to investigate the gene expression and its function. Consequently, the quality of extracted RNA is really significant. Correspondingly, this research was accomplished to optimize the RNA extraction methods and compare the amounts of tissue or quality of tissue. Relatively, the cancerous tissue of human stomach in fresh and frozen conditions and also the mouse fresh tissue were studied. Some factors like the amount of samples, efficacy differences of diverse extraction buffers (TriPure, Trizol) and also the efficacy of b-mercaptoethanol were compared and investigated. The results indicated that the less amount (1-2 mg) compared to other amounts (2-5 mg, 5-15 mg) yielded the best quality and the RNA bands (5S, 18S, 28S) were observed perfectly. Relatively, comparing and measuring some kinds of buffers (Trizol, TriPure) indicated no difference in RNA extraction quality. The last investigated factor was the effect of b- mercaptoethanol which was used along with TriPure to remove the RNAse. Conclusively, no effective impression was observed.
Indexed as
Identifiers
25815283PMC4359706W2126743155What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.