ArticleNature communications2015
End-targeting proteomics of isolated chromatin segments of a mammalian ribosomal RNA gene promoter.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
- Locus-specific proteomics identifies novel regulators of Epstein-Barr virus lytic reactivation.Journal of virology · 2026Article
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- A systematic guide for identifying transcription factors that directly regulate the expression of a gene of interest.Genome research · 2026Review
- DNA O-MAP uncovers the molecular neighborhoods associated with specific genomic loci.bioRxiv : the preprint server for biology · 2025Article
- TurboCas: A method for locus-specific labeling of genomic regions and isolating their associated protein interactome.Molecular cell · 2024Article
- Anti-gene oligonucleotide clamps invade dsDNA and downregulateMolecular therapy. Nucleic acids · 2024Article
- Zfp106 binds to G-quadruplex RNAs and inhibits RAN translation and formation of RNA foci caused by G4C2 repeats.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- POLE3 is a repressor of unintegrated HIV-1 DNA required for efficient virus integration and escape from innate immune sensing.Science advances · 2023Article
- Review
- Modification of alternative splicing in bovine somatic cell nuclear transfer embryos using engineered CRISPR-Cas13d.Science China. Life sciences · 2022Article
- Telomere-specific chromatin capture using a pyrrole-imidazole polyamide probe for the identification of proteins and non-coding RNAs.Epigenetics & chromatin · 2021Article
- TINC- A Method to Dissect Regulatory Complexes at Single-Locus Resolution- Reveals an Extensive Protein Complex at the Nanog Promoter.Stem cell reports · 2020Article
- Transcriptional suppression of ribosomal DNA with phase separation.Science advances · 2020Article
- Locus-specific chromatin isolation.Nature reviews. Molecular cell biology · 2020Article
- Purification and enrichment of specific chromatin loci.Nature methods · 2020Review
- Interactions With Histone H3 & Tools to Study Them.Frontiers in cell and developmental biology · 2020Review
- A distinct isoform of ZNF207 controls self-renewal and pluripotency of human embryonic stem cells.Nature communications · 2018Article
- Versatile and efficient chromatin pull-down methodology based on DNA triple helix formation.Scientific reports · 2018Article
- dCas9-targeted locus-specific protein isolation method identifies histone gene regulators.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Toehold-enhanced LNA probes for selective pull down and single-molecule analysis of native chromatin.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The unbiased identification of proteins associated with specific loci is crucial for understanding chromatin-based processes. The proteomics of isolated chromatin fragment (PICh) method has previously been developed to purify telomeres and identify associated proteins. This approach is based on the affinity capture of endogenous chromatin segments by hybridization with oligonucleotide containing locked nucleic acids. However, PICh is only efficient with highly abundant genomic targets, limiting its applicability. Here we develop an approach for identifying factors bound to the promoter region of the ribosomal RNA genes that we call end-targeting PICh (ePICh). Using ePICh, we could specifically enrich the RNA polymerase I pre-initiation complex, including the selectivity factor 1. The high purity of the ePICh material allowed the identification of ZFP106, a novel factor regulating transcription initiation by targeting RNA polymerase I to the promoter. Our results demonstrate that ePICh can uncover novel proteins controlling endogenous regulatory elements in mammals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.